胆固醇分子结构中红外光谱研究

Study on the molecular structure of cholesterol by mid-infrared spectroscopy

采用中红外(MIR)光谱(包括:一维MIR光谱、二阶导数MIR光谱、四阶导数MIR光谱及去卷积MIR光谱)开展了胆固醇分子结构的研究。试验表明:胆固醇分子的红外吸收模式主要包括:ν■、ν■、ν■、ν■、ν■、νC=C-胆固醇、δ■、δ■、δ■、νC-O-胆固醇和ρ■。研究表明,胆固醇分子的去卷积MIR光谱的谱图分辨能力要优于相应的一维MIR光谱、二阶导数MIR光谱和四阶导数MIR光谱。拓展了MIR光谱技术在重要的生理活性物质(胆固醇)分子结构的研究范围。

The molecular structure of cholesterol was studied by mid-infrared(MIR)spectroscopy(including one-dimensional MIR spectroscopy,second-order derivative MIR spectroscopy,fourth-order derivative MIR spectroscopy and deconvolution MIR spectroscopy).The experiment found that the infrared absorption modes of cholesterol molecules mainly include:ν■,ν■,ν■,ν■,ν■,ν■,δ■,δ■,δ■,ν■andρ■.It was found that the deconvolution MIR spectra of cholesterol molecules have better spectral resolution than the corresponding one-dimensional MIR spectra,second-order derivative MIR spectra and fourth-order derivative MIR spectra.The research scope of MIR spectroscopy in the molecular structure of important physiological active substances(cholesterol)was expanded.