摘要
Bacterial infections have become a great threat to public health in recent years.A primary lysozyme is a natural antimicrobial protein;however,its widespread application is limited by its instability.Here,we present a poly(N-isopropylacrylamide)hydrogel inverse opal particle(PHIOP)as a microcarrier of lysozyme to prolong and enhance the efficiency against bacteria.This PHIOP-based lysozyme(PHIOP-Lys)formulation is temperature-responsive and exhibits long-term sustained release of lysozyme for up to 16 days.It shows a potent antibacterial effect toward both Escherichia coli and Staphylococcus aureus,which is even higher than that of free lysozyme in solution at the same concentration.PHIOPs-Lys were demonstrated to effectively inhibit bacterial infections and enhance wound healing in a full-thickness skin wound rat model.This study provides a novel pathway for prolonging the enzymatic activity and antibacterial effects of lysozyme.
基金
This work was supported by the National Key R&D Program of China(No.2021YFA0910300)
the National Natural Science Foundation of China(Nos.22125606 and 21976198)
the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDPB2005).
