辣椒籽不溶性膳食纤维碱法提取工艺研究及其理化性质分析

Study on extraction process of insoluble dietary fiber base method of pepper seed and analysis of its physicochemical properties

以辣椒籽为材料,采用碱法提取辣椒籽不溶性膳食纤维(insoluble dietary fiber,IDF);以辣椒籽IDF提取率为指标,通过单因素试验结合响应面分析对提取工艺进行优化,确定最优提取条件,并分析最优条件下提取的IDF成分组成和微观结构。结果表明,最佳工艺条件为:NaOH浓度0.26 mol/L、碱解时间94 min、碱解温度40℃、料液比1∶18(g∶mL),此时IDF提取率可达68.01%,与理论值67.83%相比,差异不显著;IDF的持水力、持油力以及膨胀力均随目数的增加而显著下降(P<0.05);膳食纤维是辣椒籽IDF的主要组成成分,含量达80.93%,IDF具有典型的膳食纤维网状结构。以上结果说明碱法提取的辣椒籽IDF提取率高,品质优良,此工艺可有效地从辣椒籽中提取不溶性膳食纤维,该研究将为辣椒籽IDF的开发应用提供数据支撑。

Using chili seeds as materials,the insoluble dietary fiber(IDF)of chili seeds was extracted by alkali method.Taking the IDF extraction rate of pepper seeds as the index,the extraction process was optimized by univariate test combined with response surface analysis,the optimal extraction conditions were determined,and the composition and microstructure of the extracted IDF components under the optimal conditions were analyzed.Results showed that the optimal process conditions were that the NaOH concentration was 0.26 mol/L,the alkaline lysis time was 94 min,the alkaline lysis temperature was 40℃,the material-liquid ratio was 1∶18(g∶mL),and the IDF extraction rate could reach 68.01%,which was not significant compared with the theoretical value of 67.83%.The water-holding capacity,oil-holding capacity,and expansion capacity of IDF decreased significantly with the increase of mesh(P<0.05).Dietary fiber was the main component of pepper seed IDF,the content was 80.93%,and IDF had a typical dietary fiber network structure.The above results showed that the IDF extracted by the alkali method had a high extraction rate and excellent quality,and this process could effectively extract insoluble dietary fiber from pepper seeds,which would provide data support for the development and application of pepper seed IDF.