探讨药对金银花-连翘干预痤疮的网络药理学机制

A study on network pharmacological mechanism of treating acne with couplet medicines Jinyinhua-Lianqiao

目的:基于网络药理学原理探讨药对金银花-连翘干预痤疮的分子通路及作用机制。方法:应用中药系统药理学数据库与分析平台(TCMSP)检索金银花、连翘化学成分、作用靶点;通过GeneCards数据库获取痤疮差异性靶点基因,预测中药中痤疮相关的作用靶点;通过STRING平台构建蛋白质-蛋白质相互作用网络,构建“中药-成分-关键靶标-主要通路”的可视化网络图。运用DAVID数据库对筛选出的靶点基因进行基因富集分析,从而获得基因本体论(GO)生物功能及京都基因和基因组百科全书(KEGG)信号通路注释结果,进而探究中药干预痤疮的分子作用机制。结果:药对金银花-连翘干预痤疮共预测得到活性成分29个,涉及核心靶点基因97个、GO生物功能103个、KEGG信号通路119个。结论:药对金银花-连翘主要活性成分通过多靶点、多通路干预痤疮,主要涉及核受体的活性、转录因子活性等功能,以及白细胞介素(Interleukin,IL)-17信号通路、肿瘤坏死因子(Tumor Necrosis Factor,TNF)信号通路和磷脂酰肌醇3激酶(Phosphatidylinositol 3-kinase,PI3K)-丝氨酸/苏氨酸蛋白激酶(Akt Serine/Threonine Kinase,Akt)等信号通路。

Objective:Based on the principle of network pharmacology,to explore the molecular pathway and action mechanism of Jinyinhua(Flos Lonicerae Japonicae)-Lianqiao(Fructus Forsythiae)on acne.Methods:TCMSP database was used to search the chemical constituents and targets of honeysuckle and forsythia.The differential target genes of acne were obtained by GeneCards database to predict the targets related to acne in TCM.The protein-protein interaction network(PPI)was constructed by STRING platform,and the visual network diagram of TCM medicine-ingredients-key targets-main pathways was constructed.The DAVID database was used to analyze the gene enrichment of the selected target genes,so as to obtain the annotation results of GO biological function and KEGG signaling pathway,and then to explore the molecular mechanism of TCM medicine intervention on acne.Results:A total of 29 active components were predicted,involving 97 core target genes,103 biological functions and 119 signaling pathways.Conclusion:The main active components of the couplet medicines Jinyinhua-Lianqiao intervenes acne through multiple targets and multiple pathways,mainly involving the activity of nuclear receptors,transcription factor activity,and signaling pathways such as interleukin-17(IL-17)signaling pathway,tumor necrosis factor(TNF)signaling pathway and PI3K-Akt signaling pathway.