血清7种微RNA单独或联合甲胎蛋白检测对肝细胞癌的诊断价值

Values of serum 7 microRNAs alone or in combination withα-fetoprotein in diagnosing hepatocellular carcinoma

目的 分析血清7种miRNA(7miRNAs,包括miRNA-122、miRNA-192、miRNA-21、miRNA-223、miRNA-26a、miRNA-27a和miRNA-801)单独或联合甲胎蛋白(AFP)检测对肝细胞癌(HCC)的诊断价值。方法 选择2021年1月至2021年6月我院收治的249例患者为研究对象,其中163例为确诊的HCC患者(HCC组)、86例为非HCC患者(非HCC组)。检测两组患者血清7miRNAs和AFP水平,根据临床既定标准(7miRNAs≥-0.5和/或AFP≥20 ng/mL为阳性)计算2个指标单独和联合检测诊断HCC的灵敏度、特异度、阳性预测值和阴性预测值。运用ROC曲线分析2个指标单独及联合检测对HCC的诊断价值。结果 在249例患者中,HCC组患者的7miRNAs和AFP水平均高于非HCC组患者,差异均有统计学意义(P均<0.01)。血清7miRNAs、AFP和两者联合检测诊断HCC的灵敏度分别为75.46%、57.06%和87.12%,特异度分别为74.42%、94.19%和69.77%,阳性预测值分别为84.83%、94.90%和84.52%,阴性预测值分别为61.54%、53.64%和74.07%,AUC分别为0.787、0.819和0.864。结论 血清7miRNAs和AFP联合检测对HCC的诊断效能优于AFP单独检测,有更高的诊断价值。

Objective To analyze the roles of serum 7 microRNAs(7miRNAs,including miRNA-122,miRNA-192,miRNA-21,miRNA-223,miRNA-26a,miRNA-27a,and miRNA-801)alone or in combination withα-fetoprotein(AFP)in diagnosing hepatocellular carcinoma(HCC).Methods A total of 249 patients who were admitted to our hospital from Jan.2021 to Jun.2021 were enrolled,including 163 patients with confirmed HCC(HCC group)and 86 patients without HCC(non-HCC group).The serum levels of 7miRNAs and AFP were measured in the 2 groups,and based on the established clinical criteria(7miRNAs≥-0.5 and/or AFP≥20 ng/mL were positive),the sensitivity,specificity,positive predictive value and negative predictive value of the 2 indicators were calculated for the diagnosis of HCC alone and in combination.The receiver operating characteristic curve was used to analyze the diagnostic value of the 2 indicators used alone or in combination for HCC.Results Among 249 patients,the levels of 7miRNAs and AFP in the HCC group were significantly higher than those in the non-HCC group(both P<0.01).The sensitivities of serum 7miRNAs,AFP and their combination were 75.46%,57.06%and 87.12%for the diagnosis of HCC,respectively;the specificities were 74.42%,94.19%and 69.77%,respectively;the positive predictive values were 84.83%,94.90%and 84.52%,respectively;the negative predictive values were 61.54%,53.64%and 74.07%,respectively;and the area under curve values were 0.787,0.819 and 0.864,respectively.Conclusion The combined detection of serum 7miRNAs and AFP has higher diagnostic value than that of AFP used alone for HCC.