培养副鸡嗜血杆菌用干粉培养基的优化研究

Study on Optimization of Dry Powder Medium for Cultivation of Haemophilus paragallinarum

优化副鸡嗜血杆菌干粉培养基配方。通过单因素平行对比配方中碳源、血清、辅酶等营养物质,确定各个因素对副鸡嗜血杆菌生长的影响。研究筛选出了适宜副鸡嗜血杆菌生长的干粉培养基配方:鸡肉蛋白胨、酪胨、多价胨、酵母粉、葡萄糖等,再添加7.5%鸡血清和10 mg/L辅酶Ⅰ,于37℃、5%CO_(2)恒温培养箱中160 r/min振荡培养14~16 h。优化后的配方更适于培养副鸡嗜血杆菌,为副鸡嗜血杆菌疫苗大规模生产提供了参考依据。

To optimize the formula and culture process of Haemophilus paragallinarum dry powder culture medium.Through single factor parallel comparison of carbon source,serum,coenzyme and other nutrients in the formula,the influence of each factor on the growth of Haemophilus paragallinarum was determined.The dry powder culture medium formula suitable for the growth of Haemophilus paragallinarum was selected:chicken peptone,casetone,polyvalent peptone,yeast powder,glucose,etc.,and 7.5%chicken serum and 10 mg/L coenzyme I were added,and then cultured for 14~16 hours in a constant temperature incubator at 37℃and 5%CO_(2) at 160 r/min.The optimized formula is more suitable for the cultivation of Haemophilus paragallinarum,which provides a reference basis for large-scale production of Haemophilus paragallinarum vaccine.