超高效液相色谱-串联质谱法测定猪牛组织中甲基盐霉素残留量

Determination of Narasin Residues in the Tissue of Pig and Cattle by High Performance Liquid Chromatography-Tandem Mass Spectrometry

建立超高效液相色谱-串联质谱法测定猪牛组织中甲基盐霉素残留量。猪、牛组织中残留的甲基盐霉素,经乙腈溶液提取,硅胶固相萃取柱净化,超高效液相色谱-串联质谱法测定,外标法定量。甲基盐霉素在5 ng/mL~250 ng/mL的浓度范围内呈现良好的线性关系,甲基盐霉素在猪、牛肌肉、肝脏、肾脏、脂肪中的检测限均为2.5μg/kg,定量限均为5.0μg/kg,猪牛肌肉、肝脏、肾脏、脂肪中5.0 ng/g~100 ng/g添加浓度范围内的回收率均值为71.9%~88.4%,批内批间RSD值均<15%。

A method of high performance liquid chromatography-tandem mass spectrometry for determination of narasin in the tissue of pig and cattle was developed.The samples were extracted with acetonitrile,and cleaned up with silica solid phase extraction cartridg.The separation of the target was carried out on a C 18 column by elution with methanol-0.2%formic acid as mobile phase,The results showed that correlation coefficient was 0.9999.The LOD and LOQ were 2.5μg·kg-1 and 5.0μg·kg-1.The average recoveries were between 71.9%~88.4%at spiked levels of 5.0~100μg·kg-1.The RSD were below 15%.The real sample tests showed this method can be used for the sensitive and accurate determination of narasin residues in the tissue of cattle and pig.