植物靛蓝提取物的分析检测方法研究进展

Research Progress on Determination Methods of Plant Indigo Extract

以植物靛蓝提取物为研究对象,对有效成分的检测方法的国内外研究进展和发展趋势进行了综述。文章概述了植物靛蓝提取物的主要成分及其功能;介绍了高效液相色谱法、薄层色谱法、薄层扫描法、双波长分光光度法、滴定分析法、气相色谱-质谱联用法和多种检测方法联用等用于靛蓝提取物分析测试的方法,重点分析了高效液相色谱法的应用(色谱柱、流动相、检测波长和评价参数的选择);同时针对现有分析方法中存在的问题进行了分析,并展望了植物靛蓝提取物分析检测未来发展趋势。

Taking plant indigo extract as the research object,the research progress and trend of the detection methods for the main effective components at home and abroad were reviewed.The main components and their functions of plant indigo extract such as indigo and indirubin were summarized.The analytical examination methods of indigo extract were reviewed,mainly including high performance liquid chromatography,thin layer chromatography,thin layer scanning,dual wavelength spectrophotometry,titration analysis,gas chromatography-mass spectrometry and the combination of multiple detection methods.The application of high performance liquid chromatography(selection of chromatographic column,mobile phase,detection wavelength and evaluation parameters)was emphatically analyzed.At the same time,the problems existed in the present analysis methods were analyzed,and the future development trends of the indigo extract analysis and detection were predicted.