肺癌小鼠肿瘤组织IL-10的来源及其功能验证

Origin and Functional Verification of IL-10 in Tumor Tissue of Lung Cancer Mice

为了解肺癌肿瘤组织白细胞介素-10(interleukin-10,IL-10)的表达和来源,并探究IL-10对肿瘤的作用,采用MSD超敏电化学发光技术检测Lewis肺癌小鼠模型血清中IL-10的水平,使用流式细胞术分析肿瘤组织IL-10的主要来源,再通过CCK-8实验、膜联蛋白V-碘化丙啶(annexin V-PI)凋亡实验、划痕实验,检测IL-10重组蛋白对肺癌细胞增殖、凋亡、迁移的影响。结果表明:Lewis肺癌小鼠血清中IL-10水平较正常小鼠显著升高,肿瘤组织中的IL-10主要由免疫细胞产生。其中,B细胞和髓源性抑制细胞(myeloid-derived suppressor cells, MDSCs)是IL-10的主要来源,IL-10重组蛋白可以显著抑制LLC(小鼠Lewis肺癌细胞:Lewis lung carcinoma line)的增殖和迁移并促进其凋亡。综上可见,IL-10水平与肺癌密切相关,B细胞和MDSCs是产生IL-10的主要来源,IL-10可能通过激活CD8 T细胞或者直接作用于肿瘤细胞而发挥抗肿瘤作用。

To investigate the expression and source of interleukin-10(IL-10)in lung cancer tumor tissues,and to investigate the effect of IL-10 on tumors.The levels of IL-10 in the serum of Lewis lung carcinoma mouse model were detected by MSD hypersensitive electrochemiluminescence assay,the main source of IL-10 in tumor tissues was analyzed using flow cytometry,and the effects of IL-10 recombinant protein on proliferation,apoptosis and migration of lung cancer cells were examined by CCK-8 assay,annexin V-propidium iodide(annexin V-PI)apoptosis assay and scratch wound assay.The results showed that the serum levels of IL-10 were significantly increased in Lewis lung carcinoma mice compared with normal mice,and IL-10 in tumor tissues was mainly produced by immune cells,including B cells and myeloid-derived suppressor cells(MDSCs),which were the main source of IL-10,and the recombinant protein of IL-10 could significantly inhibit the proliferation and migration and promote the apoptosis of LLC(mouse Lewis lung carcinoma line).IL-10 level was closely related to lung cancer,B cells and MDSCs were the main source of IL-10 production,and IL-10 may play an antitumor role by activating CD8 T cells or directly acting on tumor cells.