光合细菌复配柠檬酸铜对移栽稻田水绵防效的研究

Control effect of photosynthetic bacteria(Rhodopseudomonas palustris)combined with copper citrate on spirogyra(Spirogyra communis)in transplanted rice fields

水绵是对水稻危害较重的低等藻类,本研究采用盆栽试验方法研究沼泽红假单胞菌复配柠檬酸铜对水绵的鲜重防效及施用后水绵中Mg^(2+)、Fe^(2+)、叶绿素含量和抗逆酶活性,同时测定了施用后水稻生长指标及抗逆酶活性。结果表明,施药后28 d,沼泽红假单胞菌复配柠檬酸铜对水绵的鲜重防效达92.17%,显著高于沼泽红假单胞菌或柠檬酸铜单独施用。与对照相比,水绵叶绿素、Mg^(2+)和Fe^(2+)含量分别显著降低60%,43%和39%,POD和SOD活性分别显著降低72%和71%,MDA含量显著升高50%。水稻叶片POD和SOD活性分别显著增加44%和104%,MDA含量显著降低51%,单株鲜重显著增加43%,分蘖数显著增加65%,株高增加5%。综上,沼泽红假单胞菌复配柠檬酸铜对水绵防效显著且对水稻安全,水绵叶绿素合成受到抑制和细胞膜过氧化作用是其对水绵防除的重要作用机理。

Spirogyra communis is an algae causing serious damage to rice growth in paddy fields.In this study,a pot experiment was performed to investigate the fresh weight control effects of Rhodopseudomonas palustris combined with copper citrate against S.communis,and the Mg ^(2+),Fe ^(2+),chlorophyll content and anti-stress enzyme activities of S.communis were measured and growth indexes and the related enzyme activity of rice plant were also studied.The results showed that the fresh weight control effect of R.palustris combined with copper citrate on S.communis was 92.17%28 days after application,which was significantly higher than that of R.palustris or copper citrate alone.Compared with the control,the contents of chlorophyll,Mg ^(2+)and Fe ^(2+)in S.communis were significantly decreased by 60%,43%and 39%;POD and SOD activities were significantly decreased by 72%and 71%,while MDA content was significantly increased by 50%.Compared with the control,POD and SOD activities in rice leaves were significantly increased by 44%and 104%;MDA content of rice leaves was significantly decreased by 51%;the fresh weight of rice leaves was significantly increased by 43%,and tiller number and plant height of rice were significantly increased by 65%and 5%.The results suggested that R.palustris combined with copper citrate had a significant control effect on S.communis and is safe for rice.Inhibition of chlorophyll synthesis and peroxidation of cell membrane in S.communis are the important prevention mechanisms.