木霉D5菌株的鉴定及其杀线活性测定

Identification of Trichoderma strain D5 and determination of its nematicidal activity

通过形态学和分子生物学方法鉴定了1株杀线木霉D5菌株的种类,并评价了其孢子悬浮液和发酵液对禾谷孢囊线虫Heterodera avenae 2龄幼虫的毒杀活性。结果表明,木霉D5菌株为绿色木霉Trichoderma viride(ITS序列登录号:OL661629;TEF序列登录号:ON241311)。浓度为1.0×10^(7)cfu/mL的D5菌株孢子悬浮液和发酵液原液处理2龄幼虫72 h后,线虫死亡率分别为85.20%和86.57%。在温度为20~80℃范围内以及pH为2~10时,发酵液均具有较高杀线活性。

The species of Trichoderma spp.D5 strain was identified by morphology and molecular biology methods,and also the nematicidal activity of its spore suspension,fermentation broth were evaluated in the present test.The results showed that the Trichoderma spp.D5 strain was identified as Trichoderma viride(ITS sequence accession number:OL661629;TEF sequence accession number:ON241311).The mortality of the second-stage juveniles of nematodes were 85.20%and 86.57%72 h after treatment with the concentration of 1.0×10^(7) cfu/mL spore suspension and fermentation broth of D5 strain,respectively.The highest nematicidal activity of fermentation broth was found at the temperature from 20 to 80℃and the pH value from 2 to 10.