6%寡糖·链蛋白可湿性粉剂与杀菌剂混用防治小麦白粉病的农药减施技术研究

Application of oligosaccharins-plant activator protein 6%WP mixed with chemical fungicide to control wheat powdery mildew for the purpose of reducing the pesticide load

为明确植物免疫诱抗剂6%寡糖·链蛋白可湿性粉剂与杀菌剂减量混用的增效作用,采用温室盆栽和田间药效试验相结合的方法,评价6%寡糖·链蛋白可湿性粉剂与2种化学杀菌剂减量混用对小麦白粉病的防治效果,并测定植物抗性相关酶活性等生理生化指标。结果表明,单独施用6%寡糖·链蛋白可湿性粉剂的田间最高防治效果仅为36.76%,但与杀菌剂减量30%混用,能明显提高戊唑醇和吡唑醚菌酯防效17.20~32.68百分点,恢复或优于2种杀菌剂推荐剂量防治水平,并且施用6%寡糖·链蛋白可湿性粉剂能提高小麦植株抗性相关防御酶超氧化物歧化酶、过氧化物酶、过氧化氢酶活性和游离脯氨酸的含量,降低丙二醛积累对小麦叶片细胞造成的损伤。因此,利用6%寡糖·链蛋白可湿性粉剂与戊唑醇和吡唑醚菌酯混用防治小麦白粉病,有明显的协同增效作用,可降低2种杀菌剂施用量约30%。

This study aims to clarify synergistic effect of the mixed use of oligosaccharins-plant activator protein 6%WP and fungicides.The control efficacy was measured by potted plant test and field efficacy trials,respectively.Also,the synergistic mechanism was analyzed using physiological and biochemical indicators such as the defense enzyme activity.The results showed that the highest field control efficacy was 36.76%using oligosaccharins-plant activator protein 6%WP alone.However,the control efficacy of 30%reduction of tebuconazole and pyraclostrobin can increase 17.20-32.68 percentage points by mixing of oligosaccharins-plant activator protein 6%WP compared to fungicides alone with 30%reduction,which equated with the recommended dosage.The activities of protective enzymes like superoxide dismutase,peroxidase and catalase increased after spraying oligosaccharins-plant activator protein 6%WP.The content of proline increased to resist pathogen infection.The accumulation of malondialdehyde reduced to relieve the damage the cells of wheat leave.Therefore,oligosaccharins-plant activator protein 6%WP can reduce dosage of tebuconazole and pyraclostrobin at 30%in control of wheat powdery mildew.