广东草地贪夜蛾不同田间种群对4种杀虫剂的抗药性监测

Monitoring of Spodoptera frugiperda(J.E.Smith)resistance to four types of insecticides in Guangdong

采用饲料浸药法测定了草地贪夜蛾对氯虫苯甲酰胺、多杀霉素、甲氨基阿维菌素苯甲酸盐、虫螨腈的相对敏感基线,并测定了广东省广州、东莞、开平、平远和阳江等5地田间草地贪夜蛾种群对氯虫苯甲酰胺、多杀霉素、甲氨基阿维菌素苯甲酸盐和虫螨腈的抗药性。结果表明:上述4种药剂对敏感草地贪夜蛾种群2龄幼虫的LC_(50)在0.037~0.300 6 mg/L之间。除平远种群对氯虫苯甲酰胺保持敏感外,其余4个种群对氯虫苯甲酰胺抗性在3.51~9.99倍之间,属低水平抗性;阳江和东莞种群对多杀霉素的抗性分别为17.62倍和17.71倍,属中等水平抗性;其他3个种群对多杀霉素抗性在4.69~7.60倍之间,属低水平抗性;除平远草地贪夜蛾种群对甲氨基阿维菌素苯甲酸盐抗性倍数为5.34倍,属低水平抗性外,其他4个草地贪夜蛾种群对甲氨基阿维菌素苯甲酸盐抗性倍数在0.39~1.08倍之间,属于敏感水平;开平草地贪夜蛾种群对虫螨腈的抗性倍数为10.77倍,属中等水平抗性;广州、东莞和阳江草地贪夜蛾种群对虫螨腈的抗性倍数在4.21~8.84倍之间,属低水平抗性;平远草地贪夜蛾种群对虫螨腈的抗性倍数为2.37倍,属敏感水平。

The relative sensitivity baseline of Spodoptera frugiperda(J.E.Smith)to chlorantraniliprole,spinosad,emamectin benzoate and chlorfenapyr was determined by artificial diet dipping bioassay method,and the resistance of S.frugiperda populations to four kinds of pesticides in Guangzhou,Dongguan,Kaiping,Pingyuan and Yangjiang in Guangdong province was determined.The results showed that the LC_(50)values of the above four insecticides on the 2nd instar larvae of the sensitive S.frugiperda population were between 0.037 mg/L and 0.3006 mg/L.The results of resistance monitoring showed that the resistance multiple of S.frugiperda population to chlorantraniliprole in Pingyuan was 1.54 times,which belonged to the sensitive level,while that in the other four places to chlorantraniliprole was 3.51-9.99 times than the sensitivity baseline,which belonged to the low level of resistance.The resistance multiples of S.frugiperda populations to spinosad in Yangjiang and Dongguan were 17.62 and 17.71 times,respectively,belonging to medium level resistance,while that in the other three places are between 4.69-7.60,which belonged to low-level resistance.Except that the population of S.frugiperda in Pingyuan was 5.34 times resistant to emamectin benzoate,which was a low-level resistance,that in other four places was 0.39-1.08 times,which was a sensitive level.The resistance multiple of S.frugiperda population in Kaiping to chlorfenapyr is 10.77 times,which belonged to medium level resistance,while that in Guangzhou,Dongguan and Yangjiang was between 4.21-8.84 times,which belonged to low-level resistance,and that in Pingyuan was 2.37 times,which belonged to the sensitive level.