流式微球技术高通量筛查中重度盆腔内异症患者细胞因子谱的变化

Change of cytokine profiles in patients with moderate and severe pelvic endometriosis in high throughput screening by cytometric bead array technology

目的观察中重度盆腔子宫内膜异位症(内异症)患者外周血和腹腔液多种细胞因子水平的变化及相互关系,探讨细胞因子谱变化在内异症发病机制中的意义。方法选取2019年3月至2021年10月该院收治的中重度盆腔内异症患者28例作为内异症组,选取同期健康体检者30例作为对照组。利用流式荧光微球高通量技术检测2组研究对象血浆或腹腔液γ干扰素(IFN-γ)、白细胞介素-1β(IL-1β)、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-12p70、IL-17A、IL-17F、IL-22、肿瘤坏死因子-α(TNF-α)、TNF-β等14种细胞因子水平,分析比较2组研究对象血浆细胞因子谱的差异、血浆各细胞因子之间的相关性,以及患者腹腔液与血浆细胞因子的相关性。结果与对照组比较,内异症组患者血浆IL-1β、IL-2水平均明显降低,IL-4、IL-6、IL-8、IL-17A、TNF-α、TNF-β水平,以及IL-17A/IL-10、IF-17F/IL-10比值均明显升高,差异均有统计学意义(P<0.05);内异症患者血浆TNFβ与IL-1β、IL-4与IL-17A、IL-22与IL-17F分别呈正相关;内异症患者腹腔液与血浆部分细胞因子具有相关性。结论中重度内异症患者血浆细胞因子谱异常,并影响腹腔液局部细胞因子水平的改变,可能在疾病的发病机制中发挥着重要作用。

Objective To observe the changes and mutual relation of multiple cytokines in peripheral blood and peritoneal fluid of the patients with moderate and severe pelvic endometriosis,and to explore the significance of the cytokine profiles changes in the pathogenesis of endometriosis.Methods Twenty-eight patients with moderate and severe pelvic endometriosis treated in this hospital from March 2019 to October 2021 were selected as the endometriosis group and 30 healthy subjects undergoing physical examination at the same time were selected as the control group.The cytometric beads array technology was used to detect the levels of 14 cytokines in plasma or peritoneal fluid,including interferon(IFN)-γ,interleukin(IL)-1β,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12p70,IL-17A,IL-17F,IL-22,tumor necrosis factor(TNF)-αand TNFβ,the difference of plasma cytokine profiles was analyzed and compared between the two groups.The correlation among various cytokines and the correlation between the peritoneal fluid and plasma cytokines was also analyzed.Results Compared with the control group,the levels of plasma IL-1βand IL-2 in the endometriosis group were significantly decreased,but the plasma IL-4,IL-6,IL-8,IL-17A,TNF-αand TNF-βlevels and IL-17A/IL-10 and IF-17F/IL-10 ratios were significantly increased,and the differences were statistically significant(P<0.05).The plasma TNFβin the patients with endometriosis was positively correlated with IL-1β,IL 4 was positively correlated with IL 17A and IL 22 was positively correlated with IL 17F;there was the correlation between peritoneal fluid and plasma partial cytokines in the patients with endometriosis.Conclusion The plasma cytokine profiles in the patients with moderate and severe endometriosis are abnormal,which affects the change of local cytokines levels in peritoneal fluid and may play an important role in the pathogensis of the disease.