马尔尼菲篮状菌毒力因子及耐药基因分布情况分析

Analysis of the Distribution of Virulence Factors and Antibiotic Resistance Genes in Talaromyces marneffei

目的:探讨马尔尼菲篮状菌毒力因子、耐药基因及双相转换基因的分布情况。方法:从NCBI数据库下载7株马尔尼菲篮状菌基因组数据,通过对MARDy、DFVF数据库和根据文献整理出双相转换基因进行同源搜索,分析潜在的毒力因子、耐药基因及双相转换基因,并比较各菌株之间的差异。结果:马尔尼菲篮状菌中总计192种毒力因子,27个双相转换基因及13个耐药基因。这些毒力因子导致皮肤感染、感染、侵袭性念珠菌病、副球孢子菌病等疾病,但各菌株毒力因子差异明显。双相转换基因检测发现,有来自其他双相转换真菌的AMY1、SID1、RYP3及DRK1基因。耐药基因检测发现,7株菌中均检测出耐氟康唑、伊曲康唑和米卡芬净的同源耐药基因。结论:马尔尼菲篮状菌毒力及双相转换机制复杂,且各菌株存在异质性,在所有菌株中均发现多种同源耐药基因,提示具有耐药风险。

Objective:To analysis the distribution of potential virulence factors,antibiotic resistance genes and dimorphism genes in Talaromyces marneffei.Methods:The genomic data of 7 strains of Talaromyces marneffei were downloaded from NCBI database,and homologous search was conducted for dimorphism genes by MARDy and DFVF databases and sorting out literatures.The potential virulence factors,antibiotic resistance genes and dimorphism genes were analyzed,and the differences among different strains were compared.Results:A total of 192 virulence factors,27 dimorphismgenes and 13 antibiotic resistance genes were found in Talaromyces marneffei.These virulence factors can cause cutaneous infections,infections,invasive candidiasis disease,paracoccidioidomycosis and other diseases,but the virulence factors of each strain were significantly different.Analysis of the dimorphism genes revealed the presence of AMY1,SID1,RYP3,and DRK1 genes,which can also be found in other dimorphic fungi.The detection of antibiotic resistance genes showed that homologous resistance genes of fluconazole,itraconazole and micafungin were detected in all the 7 strains.Conclusion:The virulence factors and dimorphism genes mechanism of Talaromyces marneffei are complex and different strains are heterogeneous.Multiple homologous antibiotic resistance genes were found in all strains,suggesting the risk of antibiotic resistance.