15个马铃薯AP2/ERF转录因子的生物信息学与表达分析

Bioinformatics and Expression Analysis of 15 Potato AP2/ERF Transcription Factors

AP2/ERF(APETALA2/ethylene-responsive factor)是植物中的一大类转录因子,其参与非生物胁迫反应、激素应答、植物生长、花发育、种子发育、损伤响应、病菌防御、耐高盐等多种生物学过程,为了初步阐明该转录因子在马铃薯胁迫响应中的功能,本研究对15个马铃薯StERF基因进行了生物信息学和表达分析。结果表明,15个马铃薯StERFs转录因子除St ERF3、StERF10、St ERF12、StERF120仅具有WLG结构域外,其余11个均具有YRG和WLG结构域,在进化上可分为3个亚家族(AP2,ERF和RAV)。对15个StERFs基因上游2000 bp启动子区的顺式作用元件分析发现,其包含ABRE、CGTCA-motif、TGACG-motif和TCAelement等响应元件。RT-qPCR分析结果表明马铃薯StERFs基因在聚乙二醇(PEG-6000)、乙烯、水杨酸(SA)、茉莉酸甲酯(MeJA)和甘露醇等处理下表达模式不同,StERF79、StERF72在5种处理下均上调表达,StERF156,StERF5在水杨酸和甘露醇胁迫处理下均上调表达。结果为进一步研究马铃薯StERFs基因的功能提供了基础。

AP2/ERF(APETALA2/ethylene-responsive factor)is a main type of transcription factor in plants,which is involved in abiotic stress response,hormone response,plant growth,flower development,seed development,injury,pathogen defense,salt tolerance and other processes.In order to preliminarily clarify the function of this transcription factor in response to potato stress.In this study,15 potato StERF genes were analyzed using bioinformatics and expression pattern.The results showed that except for the WLG domain of StERF3,StERF10,StERF12 and StERF120 of the 15 potato StERFs transcription factors,the other 11 had YRG and WLG domains.It can be divided into three subfamilies(AP2,ERF,and RAV).The result from analysis of cis-acting elements in the2000 bp upstream promoter region of the StERF genes found that they contained responsive elements such as ABRE,CGTCA-motif,TGACG-motif and TCA-element.The results of RT-qPCR analysis showed that the potato StERF genes expressed differently under the treatments of polyethylene glycol(PEG-6000),ethylene,salicylic acid(SA),methyl-jasmonate(MeJA)and mannitol.StERF79,StERF72 were up-regulated under 5 treatment factors.StERF156,StERF5 were up-regulated in response to stress treatments of salicylic acid and mannitol.The results of the study could provide a theoretical basis for further research of function of potato StERFs genes.