苦荞糖转运载体FtSWEETs的鉴定与生物信息学分析

Identification and Bioinformatics Analysis of Sugar Transporter FtSWEETs in Tartary Buckwheat

植物SWEET转运蛋白是介导糖类进行跨膜运输的重要载体。本研究基于苦荞转录组测序数据库,筛选到34个FtSWEETs基因。基因序列分析发现,苦荞FtSWEETs基因序列长度为533~2890 bp,编码氨基酸残基为51~327个。聚类分析发现,苦荞FtSWEETs被归为4类,4个属于类型I,9个属于类型Ⅱ,6个属于类型Ⅲ,11个属于类型Ⅳ。蛋白序列分析发现,29个FtSWEETs的最长开放阅读框(MaxORF)编码蛋白具有跨膜结构域。基于苦荞茎的转录组测序分析,共检测到15个FtSWEETs基因在苦荞幼苗茎中表达,包含11个差异表达基因(DEGs)。其中,4个DEGs在出苗后5 d的茎中高表达,随后急剧下降;4个DEGs在出苗后5~15 d的茎中表达量逐渐下降;1个DEG在出苗后10和15 d的茎中高表达,2个DEGs在出苗后15 d的茎中高表达,推测这可能与它们在不同发育阶段发挥特定功能有关。相关性分析发现苦荞FtSWEETs基因间存在多个显著正相关或负相关关系,说明其可能存在协同或功能互补效应。本研究为进一步探索苦荞糖转运的分子调控机制提供了依据。

Plant SWEET proteins are crucial carriers that mediate sugar transmembrane transport.In this study,a total of 34 FtSWEET genes were screened in Tartary buckwheat based on the database of transcriptome sequencing.Bioinformatics analysis indicated that the gene sequence length of FtSWEETs in Tartary buckwheat were 533~2890 bp,and the max open reading frame(MaxORF)encoding amino acids length were 51~327 aa.Cluster analysis found that FtSWEETs were classified into 4 categories,with 4 belonging to Type I,9 belonging to TypeⅡ,6belonging to TypeⅢ,and 11 belonging to TypeⅣ.Protein sequence analysis indicated that the MaxORF of 29FtSWEETs contained transmembrane domain.A total of 15 FtSWEET genes were detected expressed in the seedling stem of Tartary buckwheat,including 11 differentially expressed genes(DEGs),based on the transcriptome sequencing analysis.Four DEGs were highly expressed in stems at 5 days after seedling emergence,and then decreased sharply,four DEGs were decreased gradually in stems from 5 to 15 days after seedling emergence,one DEG was highly expressed in stems at 10 and 15 d after seedling emergence,and two DEGs were highly expressed in stems at 15 d after seedling emergence,which might be related to their specific functions at different developmental stages.Correlation analysis confirmed that there were significant positive or negative correlations among Tartary buckwheat FtSWEET genes,suggesting that the synergistic or complementary effects might be occurred among FtSWEETs genes.This study might provide a basis for further exploration of the molecular regulation mechanisms in sugar transport of Tartary buckwheat.