三十烷醇对盐胁迫下园林植物桔梗生理特性的影响

Effects of Triacontanol on Physiological Characteristics of Garden Plant Platycodon grandiflorus under Salt Stress

为了揭示三十烷醇(triacontanol,TRIA)对盐胁迫下园林植物桔梗(Platycodon grandiflorus)生理特性的影响。本研究使用300 mmol/L Na Cl对桔梗进行盐胁迫,同时对桔梗叶面喷施1、5和10μmol/L的三十烷醇溶液,共处理4次。处理15 d后,测量桔梗鲜重、干重、含水量、叶片可溶性蛋白、叶绿素含量、净光合速率。测量桔梗叶片中超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量和Na+和K+含量。结果显示,叶面喷施1、5和10μmol/L的三十烷醇溶液升高了鲜重、干重、含水量和叶片可溶性蛋白含量。叶面喷施1.0、1.5和2.0 mmol/L的亚精胺溶液升高了叶绿素含量和净光合速率。叶面喷施1、5和10μmol/L的三十烷醇溶液升高了叶片SOD和POD活性,降低了MDA含量。叶面喷施1、5和10μmol/L的三十烷醇溶液降低了叶片Na+含量和Na+/K+比值,升高了K+含量。本研究表明叶面喷施1、5和10μmol/L的三十烷醇可有效改善盐胁迫下桔梗的生理特性、光合活性、抗氧化性和Na+/K+平衡。因此,三十烷醇可能是一种提高桔梗耐盐性的候选植物生长调节剂。

In order to reveal the effects of triacontanol(TRIA)on physiological characteristics of garden plant Platycodon grandiflorus under salt stress.In this study,300 mmol/L NaCl was used to treat Platycodon grandiflorus under salt stress,and 1,5 and 10μmol/L triacontanol solution was sprayed on the leaves of Platycodon grandiflorus for 4 times.After 15 days of treatment,fresh weight,dry weight,water content,leaf soluble protein,chlorophyll content and net photosynthetic rate of Platycodon grandiflorus were measured.Superoxide dismutase(SOD)activity peroxidase(POD)activity,malondialdehyde(MDA)content,Na~+and K~+content in Platycodon grandiflorus leaves were measured.The results showed that spraying 1,5 and 10μmol/L triacontanol solution on leaves increased fresh weight,dry weight,water content and leaf soluble protein content.Spraying 1,5 and 10μmol/L triacontanol solution on leaves increased chlorophyll content and net photosynthetic rate.Spraying 1,5 and 10μmol/L triacontanol solution on leaves increased the activities of SOD and POD and decreased the content of MDA in leaves.Spraying 1,5 and 10μmol/L triacontanol solution on leaves decreased Na~+content and Na~+/K~+ratio,and increased K~+content in leaves.This study showed that foliar spraying 1,5 and 10μmol/L triacontanol could effectively improve the physiological characteristics,photosynthetic activity,antioxidant activity and Na~+/K~+balance of Platycodon grandiflorus under salt stress.Therefore,triacontanol may be a candidate plant growth regulator to improve the salt tolerance of Platycodon grandiflorus.