Optical and electrochemical dual detection ofβ-lactoglobulin based on the methylene blue@copper-based metal-organic framework

β-lactoglobulin is an effective indicator of allergic protein detection.Herein,we produced a copper-based metal-organic framework coated with methylene blue,to realize the optical and electrochemical dual detection ofβ-lactoglobulin.Methylene blue was successfully encapsulated inside the copper-based metal-organic framework and released after addition ofβ-lactoglobulin.As the concentration ofβ-lactoglobulin increased,the intensity of the ultraviolet absorption band and the response current increased with the increasing concentration of methylene blue released from the copper-based metal-organic framework.The optical detection range is from 0.10 mg/mL to 10 mg/mL,and the detection limit is 0.10 mg/mL.The electrochemical detection range is from 1.0×10^(-7) mg/mL to 8.0×10^(-7) mg/mL,the detection limit is 2.0×10^(-8) mg/mL.The dual detection strategy,with no interfere with each other,played a synergetic role in the quick qualitative and precise quantitative analyses ofβ-lactoglobulin in a wide range of applications.