SH3TC2促进结直肠癌的发生与进展——基于生信分析

SH3TC2 promotes the occurrence and progression of colon and rectal adenocarcinoma:based on bioinformatics analysis

目的:基于TCGA和GTEx样本数据信息挖掘在结直肠癌中异常表达且与预后相关的基因,以期提高其诊疗效果和改善预后生存。方法:利用GEPIA 2.0可视化分析平台对公共数据库中癌及癌旁1034个组织标本数据进行差异表达和预后生存相关mRNA筛选,使用FunRich软件通过韦恩图筛选共同差异基因并进行GO和KEGG富集分析,再通过GEPIA2.0和UALCAN数据库进行关键差异基因的表达和预后验证;接着通过TIMER2.0数据库探索关键基因的表达水平与免疫细胞浸润的相关性;最后通过STRING数据库在线进一步分析关键基因的蛋白相互作用网络。结果:共筛选到11106个差异表达基因(结肠癌5337个,直肠癌5769个),与二者预后OS及DFS相关的500个基因取交集,筛选出BGLAP、COQ2、CRLF1、PREX2、SH3TC2、PTPRM、ZNF154、HOXA4等8个表达差异基因且与预后生存DFS相关。验证后发现BGLAP、COQ2、CRLF1、PREX2、PTPRM、ZNF154、HOXA4可能在癌症的不同阶段发挥促癌、抑癌的双重功能;仅SH3TC2在结直肠癌中高表达(P<0.01),而预后生存分析表明高表达患者预后DFS显著更短(P<0.01),与预后OS无统计学差异(P>0.05);其表达水平与肿瘤免疫浸润评估显示结肠癌与CD4+T细胞浸润相关性,最高仅0.124(P=0.013),直肠癌中与免疫细胞浸润相关性无统计学意义(P>0.05);STRING数据库分析提示SH3TC2与SBF2、LITAF、MTMR2、MTMR2、GDAP1等蛋白相互作用可能性较大。结论:SH3TC2在结直肠癌中高表达,且高表达患者预后DFS更短,有可能是结直肠癌新的潜在诊断和预后DFS判断的标志物;SH3TC2表达水平在结直肠癌中与免疫细胞浸润相关性较弱。

Objective:In order to improve the diagnosis and treatment effect and improve the prognosis and survival,the abnormal expression of genes and related to prognosis is mined in clolrectal cancer based on the data information of TCGA and GTEx samples.Methods:First,visual analysis platform of GEPIA 2.0 was used to screen mRNA related to differential expression and prognosis of 1034 samples from cancer and paracancerous tissues in public database Common,differential genes were screened by W ayne diagram and enrichment analysis of GO and KEGG were performed by FunRich software.Second,the expression and prognosis of key differential genes were verified by GEPIA 2.0 and UALCAN database.Third,the correlation betwcen the expression level of key genes and immune cell infiltration was explored through TIMER 2.0 database.Finally,the protein interaction network of key genes was further analyzed online by STRING database.Results:A total of 11106 differentially expressed genes(5337 in colon cancer and 5769 in rectal cancer)were screened,and 8 differentially expressed genes such as BGLAP,COQ2,CRLF1,PREX2,SH3TC2,PTPRM,ZNF154 and HOXA4 were screened and related to prognostic survival DFS.After verification,it was found that BGLAP,COQ2,CRLF1.PREX2,PTPRM,ZNF154 and HOXA4 might play the dual functions of promoting and inbibiting cancer in different stages of cancer,only SH3TC2 was highly expressed(P<0.01)。prognostic survival analysis showed that the prognostic DFS of patients with high expression were significantly shorter(P<0.01).while the prognostic OS of patients have no significant(P>0.05)in colon and rectal adenocarcinoma.The highest correlation between CD4^(+)T cell infiltration and colon cancer was only 0.124(P<0.05),but there was no significant correlation between SH3TC2 expression and immune cell infiltration in rectal cancer(P>0.05).STRING database analysis showed that SH3TC2 might interact with SBF2,LITAF,MTMR2,MTMR2 and GDAP1 proteins.Conclusion:SH3TC2 is highly expressed,and the prognostic DFS of patients with high expression is shorter,which may be a new potential diagnosis and prognostie marker of DFS in colon and rectal adenocarcinoma,while the expression level of SH3TC2 is weakly correlated with immune cell infiltration in colon and rectal adenocarcinoma.