微波辅助复合酶提取黑米花青素的工艺及数学模型分析

Microwave-assisted Compound Enzyme Extraction of Anthocyanin from Black Rice and Its Mathematical Model Analysis

采用微波辅助-复合酶法提取黑米中的花青素。通过响应面法考察了复合酶(纤维素酶与果胶酶质量比为1∶1)用量、液料比、微波时间和微波功率对花青素得率的影响。结果表明:最佳提取条件为复合酶用量4.8 mg/g、液料比48∶1(m L∶g)、微波时间9 min、微波功率330 W,在此条件下进行黑米花青素的提取,可得花青素得率的平均值为(0.6689±0.0125)%。建立了5种数学模型对不同液料比下的黑米花青素的提取规律进行拟合,通过模型评价参数及验证试验,证实Cubic模型是描述黑米花青素提取过程的最佳数学模型。

Anthocyanins in black rice were extracted by microwave-assisted compound enzyme method.The effects of the dosage of compound enzyme(the mass ratio of cellulase and pectinase was 1∶1),the liquid-solid ratio,microwave time and microwave power,on the yield of anthocyanin were investigated by response surface method.The results showed that the optimum extraction process was dosage of compound enzyme 4.8 mg/g,liquid-solid ratio 48∶1(mL∶g),microwave time 9 min and microwave power 330 W.Under these conditions,the yield of anthocyanin was(0.6689±0.0125)%,which was close to the predicted value of 0.6742%.Five mathematical models were established to fit the extraction rule of black rice anthocyanin under different liquid-solid ratio.Through model evaluation parameters and verification tests,Cubic model was proved to be the best mathematical model to describe the extraction process of black rice anthocyanin.