4种黄山菊花品种的抗氧化和抗炎活性研究

Antioxidant and anti-inflammatory activities of four kinds of Huangshan chrysanthemum

目的探究4种黄山菊花品种水提物的抗氧化和抗炎活性。方法采用ABTS、FRAP和DPPH 3种方法测定黄山金丝皇菊、黄山皇菊、黄山贡菊和黄山黄菊4种菊花水提物的抗氧化活性,并通过NF-κB报告基因法和大鼠足肿胀模型评估其抗炎活性。结果ABTS、FRAP和DPPH结果显示,4种菊花水提物均具有抗氧化作用,其中黄山金丝皇菊作用最强,其次是黄山皇菊、黄山贡菊和黄山黄菊。NF-κB报告基因和大鼠足肿胀模型结果显示,4种菊花提取物均能够一定程度的抑制LPS诱导的NF-κB转录活性和缓解角叉菜胶致大鼠足趾肿胀程度,其中,黄山皇菊作用最为显著,其次是黄山金丝皇菊、黄山贡菊和黄山黄菊。结论4种黄山菊花品种的水提物均表现出一定的抗氧化及抗炎活性,其抗氧化活性由强到弱依次为黄山金丝皇菊、黄山皇菊、黄山贡菊和黄山黄菊,抗炎活性由强到弱依次为黄山皇菊、黄山金丝皇菊、黄山贡菊和黄山黄菊。

Objective To investigate the antioxidant and anti-inflammatory activities of four kinds of Huangshan chrysanthemum.Methods ABTS,FRAP and DPPH were used to detect the antioxidant activities of Huangshan golden silk chrysanthemum,Huangshan chrysanthemum,Huangshan gongju,and Huangshan dendranthema.Their anti-inflammatory activities were evaluated by NF-κB reporter gene assay and rat foot swelling models.Results The outcomes of ABTS,FRAP and DPPH showed that the water extracts of four kinds of chrysanthemum all had certain antioxidant activities and the activities of Huangshan golden silk chrysanthemum were strongest,followed by Huangshan chrysanthemum,Huangshan gongju,and Huangshan dendranthema.Results of NF-κB reporter gene assay and rat foot swelling models showed that four extracts of chrysanthemum morifolium could inhibit the transcription of NF-κB induced by LPS and alleviate foot swelling of rat induced by carrageenan,with the strongest activity of Huangshan chrysanthemum,followed by Huangshan golden silk chrysanthemum,Huangshan gongju,and Huangshan dendranthema.Conclusion The antioxidant activities of Huangshan golden silk chrysanthemum were strongest,followed by Huangshan chrysanthemum,Huangshan gongju,and Huangshan dendranthema.The anti-inflammatory activities of Huangshan chrysanthemum were strongest,followed by Huangshan golden silk chrysanthemum,Huangshan gongju,and Huangshan dendranthema.