藏红花素抑制NLRP3炎性小体对急进高原重度失血性休克大鼠肾脏损伤的保护作用

Protective Effect of Crocin Inhibition of NLRP3 Inflammatory Vesicles on Renal Injury in Rats in Acute Exposure to High Altitude and Severe Hemorrhagic Shock

目的研究藏红花素对急进高原重度失血性休克大鼠肾损伤的保护作用及保护机制。方法复制急进高原重度失血性休克大鼠模型并给予藏红花素。观察各组大鼠生存时间,记录不同时刻平均动脉压(mean arterial pressure,MAP)及SpO_(2),药物复苏3h后或大鼠死亡即刻(生存时间不足3h)处死大鼠留取血液。测定血清中肾功能指标和肾组织的炎性细胞因子。Western blot法检测NLRP3及caspase-1的蛋白表达情况。结果藏红花素可提高失血性休克大鼠生存率及生存时间,并改善MAP及血氧饱和度(peripheral capillary oxygen saturation,SpO_(2))。经藏红花素干预后肾功能指标及炎性细胞因子呈剂量依赖性降低,同时可呈剂量依赖性抑制肾组织中NLRP3及caspase-1蛋白表达。结论藏红花素抑制NLRP3炎性小体可能是急进高原失血性休克大鼠肾损伤的保护机制。

Objective To study the protective effect and mechanism of crocin on renal injury in rats with severe hemorrhagic shock at acute exposure to high altitude.Methods The rat model of severe hemorrhagic shock at acute exposure to high altitude was replicated and crocin was given.The survival time of rats in each group was observed,and MAP and SpO_(2) were recorded at different times.Blood was collected from the rats after 3-hour of drug resuscitation or immediately after death(survival time less than 3h).The indexes of renal function in serum and inflammatory factors in renal tissue were detected.Western blot was performed to detect the protein expression of NLRP3 and caspase-1.Results Crocin increased the survival rate and survival time of rats with hemorrhagic shock,and improved MAP and SpO_(2).After intervention with crocin,the indexes of renal function and inflammatory factors decreased in a dose-dependent manner,and the expression of NLRP3 and caspase-1 protein in renal tissue was inhibited in a dose-dependent manner.Conclusion The inhibitory effect of crocin on NLRP3 inflammatory bodies may be the protective mechanism of renal injury in rats with hemorrhagic shock at acute exposure to high altitude.