转Bt基因107杨根系分布特征

Root Distribution Characteristics of Transgenic Bt Poplar 107

以2个转BtCry1Ac基因107杨株系及其未转基因对照为材料,研究转Bt基因107杨的根系分布特征。结果表明:1)垂直方向上,2个转基因株系与CK的总根系及各径级根长密度、表面积密度、体积密度以及生物量密度上均随土层深度的增加而显著降低,在0~30 cm土层中,根长密度、根表面积密度、根体积密度及生物量密度均达到最大值,且显著高于其他土层;2)水平方向0~150 cm, 2个转基因株系与CK的总根表面积密度、总生物量密度随着距树干水平距离的增加呈现出先减小后增大的趋势;不同径级根系表面积密度、根长密度在距树干0~30 cm处达到最大值;3)2个转基因株系总根长密度、根表面积密度、根体积密度和生物量密度均小于对照,对照与转基因株系存在显著性差异,而2个转基因株系间无显著性差异;4)3个株系在根系分布中均以细根为主,且转基因株系细根径级的根长密度、根表面积密度表现为对照大于转基因株系且存在显著性差异,对照和转基因株系中根与粗根根长密度、根表面积密度无显著性差异。

Taking two transgenic Bt Cry1Ac gene 107 poplar lines and normal poplar seedling(control)root as the materials,the distribution characteristics of the transgenic poplar were studied.The results showed that 1)in the vertical direction,parameters of total root system and each diameter class decreased significantly with the increase of soil depth including the root length density(RLD),root surface area density(RSAD),root bulk density(RBD)and biomass density(BD).The RLD,RSAD,RBD and BD reached the maximum in 0-30 cm soil layer and were significantly higher than those in other soil layers.2)In the horizontal direction of 0-150 cm range,the total RSAD and total BD of the two transgenic lines and the control decreased first and then increased with the increase of the horizontal distance from the trunk.The total RSAD and RLD of different diameter classes reached the maximum at the distance of 0-30 cm away from the trunk.3)The total RLD,RSAD,RBD,and BD of the two transgenic lines were less than those of the control.There were significant differences between the control and the transgenic lines,but there was no significant difference between the two transgenic lines.4)The root distribution of the three lines was dominated by fine roots,and the RLD and RSAD of fine root of control group were higher than transgenic lines,and there were significant differences.There was no significant difference in RLD and RSAD between control and transgenic lines.