血浆中ZNF582甲基化在结直肠癌早期诊断中的可行性研究

The feasibility of plasma-based methylated ZNF582 test for early detection of colorectal cancer

目的:探讨血浆锌指蛋白582(zinc finger protein 582,ZNF582)基因甲基化检测用于结直肠癌早期诊断的可行性。方法:选择2021年1月至2022年6月在昆山市中医医院就诊的结直肠癌患者78例(直肠癌组)、结直肠息肉患者62例(结直肠息肉组)为研究对象,另选83例结肠镜检查正常者作为正常对照组。采用甲基化特异性荧光定量PCR检测血浆中ZNF582的甲基化状态,并进行统计学分析。结果:ZNF582在结直肠癌组患者血浆中的甲基化水平显著高于结直肠息肉组和正常对照组(P<0.0001)。ROC曲线分析显示,ZNF582甲基化用于诊断结直肠癌的AUC为0.879(95%CI 0.818~0.925)。ZNF582甲基化对结直肠息肉和结直肠癌的检测灵敏度分别为37.1%、73.1%,对结直肠病变的诊断特异性为90.4%。ZNF582甲基化对不同性别、分期、分化程度以及肿瘤大小的结直肠癌检测灵敏度无显著差异。结论:血浆ZNF582可以作为一种潜在的操作简便的、非侵入性结直肠癌早期诊断替代方案。

Objective:To investigate the feasibility of plasma-based methylated zinc finger protein 582(ZNF582)for early detection of colorectal cancer.Methods:From January 2021 to June 2022,the plasma of 78 colorectal cancers(colorectal cancer group),62 colorectal polyps(colorectal polyp group)and 83 normal control subjects(control group)in Kunshan Hospital of Traditional Chinese Medicine were collected.The methylation levels of ZNF582 in plasma was detected by methylation-specific fluorescence quantitative PCR,and statistical analysis was performed.Results:The methylation level of ZNF582 in plasma of colorectal cancer group was significantly higher than that in colorectal polyp group and control group(P<0.0001).The AUC of methylated ZNF582 methylation for detection of colorectal cancer was 0.879(95%CI 0.818-0.925).The sensitivities of methylated ZNF582 for detecting colorectal polyps and colorectal cancer were 37.1%and 73.1%,respectively,and the specificity of colorectal disease was 90.4%.Methylated ZNF582 had no significant difference in the detection of colorectal cancer with different gender,stage,differentiation and tumor size.Conclusion:Plasma-based methylated ZNF582 can be used as a potential non-invasive alternative for early diagnosis of colorectal cancer.