lncRNA SOX2OT在胃癌中的表达及其与预后的关系

Expression of lncRNA SOX2OT in gastric cancer and its relationship with prognosis

目的:通过检测长链非编码RNA SOX2重叠转录本(lncRNA SOX2OT)在胃癌组织中的表达,分析lncRNA SOX2OT与胃癌患者预后的关系。方法:以本院诊治的105例胃癌患者为研究对象,术中收集癌组织及癌旁正常胃黏膜,采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测组织中lncRNA SOX2OT表达水平。记录患者临床资料并随访3年,分析lncRNA SOX2OT表达水平与临床病理特征的关系。采用Kaplan-Meier法及Log rank检验分析胃癌组织lncRNA SOX2OT表达与患者生存率的关系。采用Cox回归模型分析胃癌患者预后的危险因素。结果:胃癌组织中lncRNA SOX2OT表达水平高于癌旁组织(3.49±0.57 vs.1.16±0.32,P<0.05)。lncRNA SOX2OT表达与年龄、性别、饮酒、肿瘤位置、吸烟无关(P>0.05),与TNM分期、肿瘤直径、淋巴结转移及分化程度有关(P<0.05)。生存分析显示,lncRNA SOX2OT低表达组3年生存率比高表达组高(75.51%vs.55.35%,Log rankχ^(2)=4.451,P=0.031)。Cox多因素分析证实,低分化胃癌、Ⅲ~Ⅳ期、lncRNA SOX2OT高表达是影响胃癌患者预后的危险因素(P<0.05)。结论:胃癌组织中lncRNA SOX2OT表达上调,可能参与胃癌进展,是影响胃癌预后的危险因素。

Objective:To discuss the expression of long non-coding RNA SOX2 overlapping transcripts(lncRNA SOX2OT)in gastric cancer tissues and its relationship with prognosis.Methods:A total of 105 gastric cancer patients diagnosed and treated in our hospital were selected as the research objects.During the operation,the cancer tissues and the normal gastric mucosa adjacent to the cancer were collected,and the expression level of lncRNA SOX2OT in the tissues was detected by quantitative reverse transcription-polymerase chain reaction(qRT-PCR).The clinical data of the patients were recorded and the patients were followed up for 3 years.The relationship between the expression level of lncRNA SOX2OT and the clinicopathological characteristics was analyzed.Kaplan-Meier method and Log rank test were used to analyze the relationship between the expression of lncRNA SOX2OT in gastric cancer and the survival rate of patients.Cox regression model was used to analyze the risk factors for the prognosis of gastric cancer patients.Results:The expression level of lncRNA SOX2OT in cancer tissues was significantly higher than that in adjacent tissues(3.49±0.57 vs.1.16±0.32),and the difference was statistically significant(P<0.05).The expression of lncRNA SOX2OT was not related to age,gender,drinking ratio,tumor location and smoking(P>0.05),but was related to TNM stage,tumor diameter,lymph node metastasis and degree of differentiation(P<0.05).Survival analysis showed that the 3-year survival rate of lncRNA SOX2OT low expression group was higher than that of higher expression group(75.51%vs.55.35%,Log rankχ^(2)=4.451,P=0.031).Cox multivariate analysis confirmed that poorly differentiated gastric cancer,stageⅢ-Ⅳ,and high expression of lncRNA SOX2OT were risk factors affecting the prognosis of gastric cancer patients(P<0.05).Conclusion:The expression of lncRNA SOX2OT is up-regulated in gastric cancer tissue,which may be involved in gastric cancer progression.