益气化痰祛瘀方对慢性间歇缺氧大鼠肺损伤的影响

Effects of Yiqi-huatan-quyu-decoction on Lung Injury in Rats with Chronic Intermittent Hypoxia

目的观察益气化痰祛瘀方对慢性间歇缺氧大鼠肺损伤的影响,并初步探讨其可能机制。方法24只SPF级SD大鼠随机分为正常对照组、慢性间歇缺氧模型组和益气化痰祛瘀方干预组,每组8只。除了正常对照组外,其余两组大鼠置于低氧舱中制备慢性间歇缺氧模型。4周后,益气化痰祛瘀方干预组给予益气化痰祛瘀方灌胃,正常对照组和慢性间歇缺氧模型组给予生理盐水灌胃,3组大鼠每次灌胃量均为19.2 mL/kg,每日2次,连续灌胃28 d。干预结束后,处死各组大鼠,取肺组织进行HE染色,经TUNEL法检测肺组织细胞凋亡。结果HE染色结果显示,慢性间歇缺氧大鼠模型的肺组织结构紊乱,肺泡数量明显减少,肺泡腔扩大,甚至破坏融合成肺大疱,肺间质炎性细胞浸润增多,肺血管壁增厚;益气化痰祛瘀方干预组大鼠肺损伤程度较慢性间歇缺氧模型组大鼠明显减轻。TUNEL染色结果显示,慢性间歇缺氧模型组大鼠肺组织细胞凋亡指数高于正常对照组(P<0.05),而益气化痰祛瘀方干预组则较慢性间歇缺氧模型组明显降低(P<0.05)。结论益气化痰祛瘀方能够减轻慢性间歇缺氧大鼠肺组织损伤程度,其机制可能与降低肺组织细胞凋亡水平相关。

Objective To observe the effects of yiqi-huatan-quyu-decoction on lung injury in chronic intermittent hypoxia rats,and explore its mechanism.Methods Twenty-four SD rats were divided into normal control group,hypoxia model group and Chinese medicine intervention group randomly,8 rats in each group.Rats of hypoxia model group and Chinese medicine intervention group were placed in a hypoxic chamber to make chronic intermittent hypoxia model.After 4 weeks,the rats in the Chinese medicine intervention group were taken yiqi-huatan-quyu-decoction by intragastric administration,while the rats in the normal control group and hypoxia model group were given saline.All three groups of rats were given 19.2 mL/kg per gavage,twice a day for 28 d.After the intervention,the rats in each group were executed and removed the lung tissues then stained the lung tissues with Hematoxylin-eosin,and the TUNEL method was used to detect apoptosis in the tissue.Results Hematoxylin-eosin staining showed that the structure of rat lung tissues in the chronic intermittent hypoxia model group were disorder,the number of alveolar was significantly reduced,the alveolar space was enlarged and even destroyed and fused into pulmonary blisters,the infiltration of inflammatory cells in the pulmonary interstitial was increased and the walls of pulmonary vessels were thickened.Compared with the model group,the degree of lung damage in yiqi-huatan-quyu-decoction intervention group rats was significantly reduced.The result of TUNEL showed that the apoptotic index of lung tissue in the chronic intermittent hypoxia model group was higher than the normal control group(P<0.05),while the index in yiqi-huatanquyu-decoction intervention group was significantly lower than the model group(P<0.05).Conclusion Yiqi-huatan-quyu-decoction reduce the degree of lung tissue damage in chronic intermittent hypoxic rats,and its mechanism may be related to reducing the level of apoptosis in lung tissue.