微小染色体维持蛋白3(MCM3)在胃癌组织中的表达及临床意义

Expression and clinical significance of minichromosome maintenance protein 3(MCM3) in gastric cancer tissues

目的研究微小染色体维持蛋白3(MCM3)在人胃癌中表达水平及临床意义。方法使用TCGA,CCLE,HPA等公共数据库分析MCM3的mRNA及蛋白在胃癌及癌旁正常组织中的表达水平。回顾性分析69例胃癌患者的临床病理特征,用免疫组织化学染色法检测肿瘤组织及癌旁正常组织中MCM3蛋白表达水平,分析其与临床病理特征间的关系。使用STRING数据库分析MCM3蛋白的相互作用网络。结果MCM3的mRNA及蛋白在胃癌组织中表达水平明显高于癌旁正常组织(P<0.05),且其高表达与胃癌肿瘤的大小相关(P<0.05)。在胃癌组织中,MCM3表达与增殖细胞核抗原(PCNA)表达水平具有相关性(R=0.61,P<0.01),并且两者之间可能存在蛋白-蛋白相互作用。结论MCM3在胃癌中通过与PCNA相互作用而发挥重要作用,并有望成为一个新的诊断及治疗靶点。

Objective To study the expression level and clinical significance of minichromosome maintenance protein 3(MCM3)in human gastric cancer.Methods The expression levels of MCM3 mRNA and protein in gastric cancer and adjacent normal tissues were analyzed using public databases such as TCGA,CCLE and HPA.The clinicopathological features of 69 patients with gastric cancer were retrospectively analyzed,and the expression levels of MCM3 protein in tumor tissues and adjacent normal tissues were detected by immunohistochemical staining,and the relationship between it and clinicopathological features was analyzed.The interaction network of MCM3 proteins was analyzed using the STRING database.Results The expression levels of MCM3 mRNA and protein in gastric cancer tissues were significantly higher than those in adjacent normal tissues(P<0.05),and its high expression was correlated with the size of gastric cancer tumors(P<0.05).In gastric cancer tissue,the expression of MCM3 was correlated with the expression level of proliferating cell nuclear antigen(PCNA)(R=0.61,P<0.01),and there may be a protein-protein interaction.Conclusions MCM3 plays an important role in gastric cancer by interacting with PCNA,and it is expected to become a new diagnostic and therapeutic target.