聚多巴胺改性胶原膜复合材料的构建、表征及其对软骨细胞增殖的影响

Preparation and characterization of polydopamine modified collagen membrane for articular cartilage repair

目的考察聚多巴胺改性胶原膜复合材料对软骨组织的黏合力及对软骨细胞增殖的影响,进一步探讨其在自体软骨细胞移植中应用的可能性。方法制备胶原膜材料,通过吸附法构建聚多巴胺改性的胶原膜复合材料。通过红外光谱仪、热重分析仪、差示热分析仪、扫描电镜和X射线光电子能谱仪等分别对复合材料的热稳定性、热学性质、多孔结构和表面元素组成等理化性质和结构特征进行表征;通过力学试验机测试聚多巴胺改性的胶原膜与新鲜软骨组织间的黏附力;通过体外细胞培养方法考察复合材料对兔软骨细胞黏附和增殖的影响。结果复合材料结构和表面元素组成随聚多巴胺吸附时间的增加而变化,聚多巴胺吸附量随复合时间延长而增加;聚多巴胺的复合对胶原膜材料的热稳定性和热学性质无明显的影响;复合材料对软骨组织的黏附力随聚多巴胺吸附量的增加而增强;复合材料对软骨细胞具有时间相关性地促进作用。结论聚多巴胺改性胶原膜复合材料在关节软骨修复中具有潜在的应用价值,但用于关节软骨修复之前,需要更多的研究来优化复合材料。

Objective To investigate the adhesion of polydopamine-modified collagen membrane composites to cartilage tissues and the effect on chondrocyte proliferation,and further explore the possibility of their application in autologous chondrocyte transplantation.Methods Porous collagen membranes were prepared,and the polydopamine-modified collagen membrane composites were constructed by the adsorption method.The physical and chemical properties and structural characteristics of the membranes,such as thermal stability,thermal properties,porous structure,and surface element composition,were characterized by infrared spectroscopy,thermogravimetric analysis,differential thermal analysis,scanning electron microscopy,and X-ray photoelectron spectroscopy,respectively.The adhesion between the polydopamine-modified collagen membrane and fresh cartilage tissue was tested by a mechanical testing machine.The effects of the membranes on the adhesion and proliferation of rabbit chondrocytes were investigated by in vitro cell culture.Results The structure and surface element composition of the membranes altered with the increase in the adsorption time of polydopamine,and the capacity of polydopamine increased with the increase in the adsorption time.The thermal stability and thermal properties of collagen membrane materials were not significantly affected by the adsorption of polydopamine.The adhesion of the membrane to cartilage tissue increased with the increase in the amount of absorbed polydopamine.The membranes showed a time-dependent promoting effect on the proliferation of the chondrocytes.Conclusions The polydopamine-modified collagen membrane has potential application in articular cartilage repair,but more research is required to optimize the membrane before it is used in articular cartilage repair.