PVA/HA复合栓塞微球的制备与理化性能研究

Preparation and physicochemical properties of PVA/HA composite microspheres for embolization

目的制备聚乙烯醇(PVA)/羟基磷灰石(HA)复合栓塞微球,并对其理化性能进行研究。方法将PVA与HA的混合溶液作为分散相、含有失水山梨糖醇脂肪酸酯的液体石蜡作为连续相,以戊二醛为交联剂采用反相悬浮化学交联法,制备PVA/HA复合栓塞微球。采用光学显微镜和扫描电镜对PVA/HA复合栓塞的外观形貌、粒径分布以及微观形貌进行观察,采用傅里叶红外光谱对PVA/HA复合栓塞微球的化学结构进行表征,并对PVA/HA复合栓塞微球的弹性以及载药和释药等性能进行测试。结果PVA/HA复合栓塞微球为内部多孔状圆形小球,粒径分布为50~300μm,弹性性能为(13.6±0.145)kPa,为PVA微球的2.28倍,载药量达(76.80±1.22)mg/g,包封率为(38.4±12.7)%,7 d内最高累积释放率为(7.37±0.101)%,最高累积释药量为(256.2±9.8)μg。结论PVA/HA复合栓塞微球具有良好的机械性能和载药释药性能,为其作为医疗器械提供了重要参考依据。

Objective To prepare polyvinyl alcohol(PVA)and hydroxyapatite(HA)composite embolization microspheres and investigate their physicochemical properties.Methods PVA/HA composite embolization microspheres were prepared by reverse suspension polymerization,using PVA and HA as dispersed phases,liquid paraffin containing sorbitan fatty acid ester as the continuous phase,and glutaraldehyde as the cross-linking agent.The morphology,particle size distribution,and microscopic morphology of PVA/HA composite embolization microspheres were observed by optical microscopy and scanning electron microscopy.The chemical structure of PVA/HA composite embolization microspheres and the elasticity,drug loading,and drug release properties of PVA/HA composite bolus microspheres were characterized by Fourier infrared spectroscopy.Results The PVA/HA composite embolization microspheres were internal,porous round spheres with a particle size distribution of 50-300μm.The elastic properties of PVA/HA composite embolization microspheres were(13.6±0.145)kPa,which was 2.28 times that of PVA microspheres,and the drug loading capacity and encapsulation efficiency were(76.80±1.22)mg/g and(38.4±12.7)%,respectively.The maximum cumulative release rate of the microspheres within 7 days was(7.37±0.101)%,and the maximum cumulative release was(256.2±9.8)μg.Conclusions PVA/HA composite embolization microspheres have good mechanical properties and drug-loading and drug-releasing properties,which provide an important reference for their use as medical devices.