多聚ADP核糖聚合酶14在甲状腺癌中的表达及临床意义

Expression level and clinical significance of PARP14 in thyroid carcinoma

目的研究多聚ADP核糖聚合酶14(PARP14)在甲状腺癌中的表达情况及与甲状腺癌患者临床病理特征的关系,并评估PARP14在甲状腺癌进展中的作用。方法使用基因表达交互分析(GEPIA)数据库分析PARP14在正常甲状腺组织和甲状腺癌人群中的表达情况以及与患者无病生存期的关系,通过免疫组化实验评估甲状腺癌患者的肿瘤组织及癌旁组织中PARP14的表达。根据染色强度,将患者分为高表达组和低表达组,分析PARP14的表达情况与临床病理特征的相关性。通过克隆形成实验和MTT实验探究PARP14对甲状腺癌细胞增殖的影响。结果生物信息学分析和免疫组织化学结果均表明PARP14在甲状腺癌组织中高表达,且高表达患者的无病生存率明显偏低。PARP14表达水平与肿瘤分期和甲状腺内扩散具有相关性,且有统计学意义(均P<0.05)。克隆形成实验和MTT实验结果表明,KIF4A的表达能促进甲状腺癌细胞的增殖(P<0.05)。结论PARP14在甲状腺癌中高表达,并与患者的临床病理特征相关,提示其可能是甲状腺癌的治疗靶点。

Objective To investigate the expression level of poly ADP ribose polymerase 14(PARP14)in thyroid cancer and its relationship with the clinicopathologic characteristics of the patient with thyroid cancer and evaluate the role of PARP14 in the progression of thyroid cancer.Methods The gene expression interaction analysis(GEPIA)database was used to analyze the expression of PARP14 in normal thyroid tissue and thyroid cancer tissue and its relationship with disease-free survival of patients.The expression of PARP14 in thyroid cancer tissue and adjacent tissues of the patient with thyroid cancer was evaluated by immunohistochemistry.According to the staining intensity,the patients were divided into the high expression group and the low expression group,and the correlation between the expression of PARP14 and clinical pathological characteristics was analyzed.The effect of PARP14 on the proliferation of thyroid cancer cells was investigated by clone formation testing and MTT testing.Results The results of bioinformatics analysis and immunohistochemistry showed that PARP14 was overexpressed in thyroid cancer tissue,and the disease-free survival rate of the patient with high expression was lower.The expression level of PARP14 was correlated with tumor stage and intrathyroidal spread(all P<0.05).The results of the clonogenic assay and the MTT assay showed that the expression of KIF4A could promote the proliferation of thyroid cancer cells(P<0.05).Conclusions PARP14 is highly expressed in thyroid cancer and is related to the clinicopathological characteristics of patients,suggesting that it may be a therapeutic target for thyroid cancer.