基于网络药理学和生物信息学探究灵芝在Ⅱ型糖尿病中的作用机制

Study on the Mechanism of Ganoderma lucidum in TypeⅡDiabetes Based on Network Pharmacology and Bioinformatics

目的:筛选灵芝改善Ⅱ型糖尿病关键成分、通路以及靶点。方法:基于GEO数据库中4个Ⅱ型糖尿病相关芯片分析结果,以及GeneCards和CTD数据库筛选结果,得到疾病靶点;使用TCMSP、HERB、ETCM和Batman-TCM数据库检索灵芝相关靶点,进而与疾病靶点取交集,得到共同靶点;将共同靶点及其相应的活性成分构建网络关系。结果:Lucialdehyde C、Lucidumol A、Ganodermanondiol、Ganoderiol F和Ganoderan B可能为灵芝改善Ⅱ型糖尿病的主要活性成分;cAMP通路、MAPK通路、C型凝集素样受体通路、AMPK通路以及胰岛素通路可能是灵芝改善Ⅱ型糖尿病的关键通路;MAPK3、PIK3CA、RELA、CALM1、INS、IL1B以及PTGS2可能是灵芝改善Ⅱ型糖尿病的关键靶点。结论:灵芝通过多成分、多靶点、多通路的调控模式,在Ⅱ型糖尿病的治疗中发挥重要作用。

Objective:To screen the key components,pathways and targets of Ganoderma lucidum to improve typeⅡdiabetes.Methods:Based on the analysis results of four typeⅡdiabetes related chips in the GEO database,as well as the screening results of GeneCards and CTD databases,disease targets are obtained;TCMSP,HERB,ETCM and Batman-TCM databases are searched for Ganoderma lucidum-related targets,and then related to disease targets.Take the intersection to get the common target;build the network relationship between the common target and its corresponding active components.Results:Lucialdehyde C,Lucidumol A,Ganodermanondiol,Ganodriol F,Ganoderan B may be the main active components of Ganoderma lucidum to improve typeⅡdiabetes;cAMP pathway,MAPK pathway,C-type lectinlike receptor pathway,AMPK pathway and insulin pathway may be the key pathway for Ganoderma lucidum to improve typeⅡdiabetes;MAPK3,PIK3CA,RELA,CALM1,INS,IL1B,PTGS2 may be the key targets of Ganoderma lucidum to improve typeⅡdiabetes.Conclusion:Ganoderma lucidum plays an important role in the treatment of type II diabetes through the regulation mode of multiple components,multiple targets and multiple signaling pathways.