肿瘤类器官技术及其在耐药研究中的应用

Research progress of tumor organoids and their application in drug resistance research

类器官,作为自问世以来便飞速发展并在各大实验平台广泛应用的三维结构微器官,其相比于传统的实验模型如2D细胞、人源性组织异种移植(patient-derived xenografts,PDX)等,更具有与真实器官较为相似的复杂结构以及多种细胞形态,并能较大程度地契合来源组织或器官的生理机能。此外,随着类器官培养技术及分析手段在研究中的创新改进,日益丰富的培养介质方案及高通量筛选等方法的建立使得类器官的应用更趋完备化和多样化。同时,新兴的类器官技术在肿瘤研究中扮演重要角色,尤其在药物筛选、疾病建模等领域可作为可靠度极高的实验模型。本篇综述概括了近年来肿瘤类器官在培养、分析方法上的部分改进和细化,以及肿瘤类器官在发展应用中相比于传统实验模型的优势,最后总结了肿瘤类器官在耐药性应用中的研究进展。本文对肿瘤类器官的耐药性概述将有利于为肿瘤耐药研究提供较为可靠的理论依据和方法参考。

Organoid,as a three-dimensional structure micro organ that has been rapidly developed since its birth and widely used in various experimental platforms,compared with traditional experimental models such as 2D cells and patient derived xenografts(PDX),it has more complex structures and multiple cell morphologies similar to real organs,and can more closely match the physiological functions of the source tissues or organs.In addition,with the innovation and improvement of organ like culture technology and analysis means in research,the establishment of increasingly rich culture medium schemes and high-throughput screening methods makes the application of organ like organisms more complete and diversified.At the same time,the emerging organ like technology plays an important role in tumor research,especially in drug screening,disease modeling and other fields,which can be used as highly reliable experimental models.This review summarizes some improvements and refinements in culture and analysis methods of tumor like organs in recent years,as well as the advantages of tumor like organs compared with traditional experimental models in development and application,and finally summarizes the research progress of tumor like organs in drug resistance application.The overview of drug resistance in tumor like organs in this paper will be helpful to provide a more reliable theoretical basis and methodological reference for the study of tumor drug resistance.