颈动脉粥样硬化患者血小板功能的变化

Platelet function in patients with carotid atherosclerosis

目的:检测颈动脉粥样硬化(CAS)患者血小板功能的变化。方法:用流式细胞术测定97例CAS和43例对照组患者外周血血小板膜糖蛋白Ⅱb-Ⅲa复合物的α亚单位(CD41)、P-选择素(CD62p)和溶酶体膜蛋白53(CD63)的表达以及血小板与淋巴、单核和中性粒细胞的凝集性。结果:与对照组相比,CAS组CD62p和CD63阳性百分率升高(P<O.05),淋巴、单核和中性粒细胞CD41阳性百分率和平均荧光强度无变化;Spearman相关分析未发现CD62p和CD63与CAS程度有关;Logis-tic多元回归分析证实CD63是发生CAS的独立危险因素(P<0.05)。结论:CAS患者血小板功能发生变化,血小板激活在CAS发病中起一定作用。

Objective:To assay the changes of platelet function in patients with carotid atherosclerosis (CAS). Methods: By flow cytometry ,the expression of platelet membrane glycoprotein Ⅱ b-Ⅲ a complex α-subunit (CD41) ,P-selectin (CD62p) , lysosomes membrane protein 53 (CD63) and platelet-lymphocyte,monocyte,neutrophil aggregation were measured in 97 CAS patients and 43 age,sex-match control patients. Results:Compared to the control,the positive percentage of CD62p and CD63 were elevated in CAS group (P<0. 05),the positive percentage and mean fluorescence intensity of CD41 on lymphocytes, monocytes, neutrophils had no significant change. Spearman correlation analysis confirmed there was no significant correlation between the expression of CD62p,CD63 and the degree of CAS. Logistic regression analysis revealed CD63 was an independent risk factor in CAS(P<0. 05). Conclusion: There are changes of platelet function in CAS,and platelet activation plays a role in CAS.