CD11b基因rs4597342位点多态性与川崎病的相关性研究

Correlation between rs4597342 polymorphism of CD11b gene and Kawasaki disease

目的探讨CD11b基因rs4597342位点多态性与川崎病(KD)及其冠状动脉损伤(CAL)的关系。方法选择株洲市中心医院儿科2019年1月1日至2021年12月30日136例KD患儿(KD组)及同期74例健康儿童(对照组)。KD患儿根据有无CAL分为KD合并冠脉损伤组(KD-CAL组,n=25)和无冠脉损伤组(KD-NCAL组,n=111)。基于限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术结合Sanger测序法检测KD组患儿与对照组儿童的CD11b基因rs4597342位点多态性,χ2检验比较KD组和对照组,及KD-CAL与KD-NCAL组样本间rs4597342基因型及等位基因频率分布差异。结果KD组CD11b基因rs4597342位点TT、TC、CC基因型分布频率分别为5.89%、47.79%、46.32%,T、C等位基因频率分别为29.78%,70.22%;健康组CD11b基因rs4597342位点TT、TC、CC基因型分布频率分别为1.35%、50.00%、48.65%,T、C等位基因频率分别为26.35%、73.65%。两组间基因型及等位基因频率分布比较,均差异无统计学意义(χ2=2.40,0.55;均P>0.05)。KD-CAL组CD11b基因rs4597342位点TT、TC、CC基因型分布频率分别为4.00%,48.00%,48.00%,T、C等位基因频率分别为28.00%,72.00%;KD-NCAL组CD11b基因rs4597342位点TT、TC、CC基因型分布频率分别为6.31%,47.75%,45.94%;T、C等位基因频率分别为30.18%,69.82%,两组间比较,均差异无统计学意义(χ^(2)=0.20,0.09;均P>0.05)。结论CD11b基因rs4597342位点多态性与KD及其CAL的发生无明显相关性。

Objective To investigate the correlation between the rs4597342 polymorphism of the CD11b gene and Kawasaki disease(KD)and coronary artery lesions(CAL).Methods From January 1,2019,to December 30,2021,a total of 136 children with KD and 74 healthy children were selected from the Pediatrics Department of Zhuzhou Central Hospital.KD children were divided into KD with CAL group(KD-CAL group,n=25)and without CAL group(KD-NCAL group,n=111).The rs4597342 polymorphism of CD11b gene was detected by polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP)combined with Sanger sequencing method.The genotype and allele frequency distribution of rs4597342 between KD and control groups,as well as between the KD-CAL and KD-NCAL groups were compared by using theχ^(2)test.Results The TT,TC,and CC genotype distribution frequencies at rs4597342 of the CD11b gene in the KD group were 5.89%,47.78%,and 46.32%,respectively,and the T and C allele frequencies were 29.78%,70.22%,respectively.The TT,TC,CC genotype distribution fre-quencies at rs4597342 of the CD11b gene in the healthy group were 1.35%,50.00%,48.65%,respectively,and the T and C allele frequencies were 26.35%,73.65%,respectively.There were no significant differences in genotype and allele frequency distribution between the two groups(χ^(2)=2.40,0.55,all P>0.05).The TT,TC,and CC genotype distribution frequencies at rs4597342 of CD11b gene in KD-CAL group were 4.00%,48.00%and 48.00%,respectively,and the frequencies of T and C alleles were 28.00%,72.00%,respectively.TT,TC,and CC genotype distribution frequencies at rs4597342 of CD11b gene in KD-NCAL group were 6.31%,47.75%and 45.94%,respectively,and T and C allele frequencies were 30.18%,69.82%,respectively.There were no significant differences between the two groups(χ^(2)=0.20,0.09,all P>0.05).Conclusions The rs4597342 polymorphism of CD11b gene and KD or its complication CAL are not significantly correlated.