AL型三系交小麦不育相关基因dCAPs标记开发与应用

Development and Application of dCAPs Markers Related to Sterility Genes in AL Type Three-line Hybrid Wheat

为快速、准确鉴别出不育系中混入的保持系种子数量,确保不育系种子繁殖纯度。本研究以3对育性稳定的不育系和其同型保持系为试验材料,采用Illumina wheat 90K芯片技术,对其SNP差异位点进行分析,设计了与不育基因相关的SNP引物,在此基础上开发了dCAPs标记,并采用杂交小麦杂交种和恢复系为检测材料对d CAPs分子标记的可靠性进行了验证。结果表明,从3对不育系和其同型保持系材料中获得一个共有SNP差异位点。根据差异位点开发了含有内切酶XmnⅠ的dCAPs标记,对含有不育基因材料进行dCAPs标记检测,表明该标记为AL型三系杂交小麦不育基因检测较理想的分子标记,为杂交小麦遗传基础研究及种子纯度检测提供了可靠工具。

Quickly and accurately identify the purity of the wheat sterile line seeds is an important part of the reproduction of the sterile line.In this study,3 pairs of sterile lines with stable fertility and their isotype maintainers were used as experimental materials.Illumina Wheat 90K array was used to analyze the SNP differential loci,the SNP primers related to infertility genes were designed,and dCAPs markers were developed.Use wheat hybrids and restorer lines to verify the detection reliability of the dCAPs molecular marker.The results showed that a common SNP difference site was obtained from the analysis of the three pairs of sterile lines and its isotype maintainer materials.The dCAPs marker containing the endonuclease XmnⅠwas developed based on the difference loci,and the dCAPs marker was tested on the material containing the sterility gene,which showed that the dCAPs marker of sterile related gene is suitable for the research and detection of sterile genes in AL type three-line hybrid wheat.It provides a reliable tool for the research on the genetic basis of hybrid wheat and the detection of seed purity.