基于SSR标记的吊瓜品种(系)DNA指纹图谱库的构建

Construction of DNA Fingerprint Library of Cantaloupe Cultivars(Lines)Based on SSR Markers

为建立吊瓜品种的DNA指纹图谱,实现对吊瓜品种的高效鉴定,本研究以5份性状稳定的吊瓜品系为供试材料,开展多态性SSR分子标记引物筛选,并构建吊瓜品系的DNA指纹图谱。结果表明,从54对SSR引物中筛选出的4对核心引物,平均每对SSR核心引物扩增的多态性条带数为7.5条,多态性信息含量(PIC)平均为0.53,5份吊瓜材料间的相似系数为0.2~0.83。利用这4对引物分别构建5份供试吊瓜唯一的DNA指纹图谱,最终确定5份材料均为不同的品系。该研究表明SSR分子标记可于构建吊瓜品种的DNA指纹图谱,为开展吊瓜品种鉴定提供技术支持,同时也有助于对吊瓜新品种的申报和保护。

To establish DNA fingerprints and efficient identification of cantaloupe(Trichosanthes laceribractea Hayata)cultivars,screening of the simple sequence repeat(SSR)molecular marker markers were carried out based on five sta ble T.laceribractea Hayata cultivars.The results showed that four core pairs of SSR primers were screened out from 54 pairs,the average number of polymorphic bands amplified by the four core primers was 7.5,and the average polymorphic information content(PIC)was 0.53,the similarity coefficient between the five cultivars was 0.2~0.83.The 4 pairs of primers were used to construct the unique DNA fingerprint of five T.laceribractea Hayat cultivars,and the 5 materials were determined to be different cultivars(lines).These results indicated that SSR molecular markers can be applied to the construction of DNA fingerprints of T.laceribractea Hayata varieties which can provide technical support for the identification of T.laceribractea Hayata varieties,facilitating its declaration and protection.