蛋白质组学技术在弱精子症中的应用进展

Advances in proteomic technologies for asthenozoospermia

弱精子症是一种精子运动活力不足的病症,约有27.8%的男性不育由弱精子症导致,其病因复杂,发病机制尚不明确,探讨其发病机制有助于寻找有效预防和治疗策略。近年来,随着蛋白质组学技术(双向电泳、酵母双杂交技术、质谱等技术)的发展,众多研究者将研究重点放在基于蛋白质组学的弱精子症研究上,揭示了弱精子症差异蛋白及所在生物通路,为弱精子症的机制研究提供了众多理论支撑。本文围绕蛋白质组学的相关技术,对弱精子症的差异蛋白表达、病因和发生机制等方面取得的诸多成果进行综述,希望为弱精子症的诊治提供新思路。蛋白质间的相互作用和通路及代谢异常为研究弱精子症的精子运动活力提供了一个很有潜力的方向。

Asthenozoospermia is a condition of deficient motility of spermatozoa,and about 27.8%of male infertility results from asthenozoospermia.Its etiology is complex and its pathogenesis is still unclear,and exploring its pathogenesis can help to find effective prevention and treatment strategies.In recent years,with the development of proteomic technologies(two-dimensional electrophoresis,yeast two-hybrid technology,mass spectrum,etc.),many researchers have focused their researches on the study of asthenozoospermia based on proteomics,revealing the differential proteins of asthenozoospermia and the biological pathways that underlie them,providing numerous theoretical support for the mechanistic study of asthenozoospermia.In this review,we focus on the relevant techniques of proteomics and summarize the many achievements made in understanding the differential protein expression,etiology,and mechanism of asthenozoospermia,which will hopefully provide new insights into the diagnosis and treatment of asthenozoospermia.Protein-protein interactions and pathways and metabolic abnormalities provide a promising direction to study sperm motility in asthenozoospermia.