PPP2R2A在非小细胞肺癌组织的表达及其临床意义

Expression of PPP2R2A and its clinical significance in non-small cell lung cancer

目的检测非小细胞肺癌(NSCLC)中PPP2R2A的表达,探讨NSCLC的临床病理学特性与其表达之间的关系。方法应用反转录-聚合酶链反应(RT-PCR)方法检测37例NSCLC肿瘤组织,及其相应的癌旁组织中PPP2R2A的表达,并分析其与临床病理特征之间的关系。结果RT-PCR检测显示PPP2R2A的相对表达量在NSCLC组织、癌旁正常组织分别为1.15±0.38、3.78±1.77差异有统计学意义(P<0.01);PPP2R2A的低表达与患者的临床病理学特征如年龄、性别、TNM分期及有无淋巴结转移无明显相关(P>0.05),但是和肿瘤的病理类型、分化程度显著相关(P<0.05)。结论PPP2R2A在非小细胞肺癌中的低表达,有助于判断非小细胞肺癌的生物学特性。

Objective To investigate the expression level of PPP2R2A in non-small cell lung cancer(NSCLC)and its correlation with the clinical significance of NSCLC.Methods Quantitative reverse transcriptase-polymerase chain reaction(RT-PCR)analysis was used to examine the expression of PPP2R2A in 37 pairs of NSCLC tissues and matched normal tissues.Chi-square test was used to analyze the correlation between miR-136 expression level and clinical features.Results The results of quantitative RT-PCR revealed that the relative expression of PPP2R2A in NSCLC tissues and matched normal tissues was 1.15±0.38 and 3.78±1.77 respectively(P<0.01).The low expression of PPP2R2A in NSCLC tissues was not correlated with the characteristics of patients with NSCLC including age,gender,TNM stage and lymph node metastasis(P>0.05),but significantly with pathological types and tumor differentiation of NSCLC(P<0.05).Conclusion The low expression of PPP2R2A in NSCLC may be beneficial to the evaluation of biological characteristics of NSCLC.