MMP14单克隆抗体对口腔鳞状细胞癌增殖、凋亡的影响

Effects of MMP14 monoclonal antibody on proliferation and apoptosis of oral squamous cell carcinoma

[目的]探究MMP14单克隆抗体对口腔鳞状细胞癌细胞增殖、凋亡的影响。[方法]构建MMP14敲低(Kd-TCa8113)、过表达(Kd-TCa8113)细胞株,与野生型TCa8113(Wt-TCa8113)对比,考察MMP14对TCa8113细胞增殖、凋亡的影响。用不同浓度的MMP14单克隆抗体与TCa8113共培养,以探讨MMP14单克隆抗体对TCa8113细胞增殖、凋亡的影响。[结果]以Wt-TCa8113为对照组,Kd-TCa8113组、Kd-TCa8113组的细胞培养48 h的增殖率分别为74.55%、114.79%,P均<0.05。流式细胞术结果显示,Wt-TCa8113、Kd-TCa8113和Oe-TCa8113培养48 h后的细胞凋亡率分别为3.47%±0.36%、33.68%±7.33%和1.60%±0.22%,P均<0.05。用50μg/mL、100μg/mL和200μg/mL浓度的抗体处理TCa8113细胞24 h后,以PBS处理组为对照,细胞增殖率分别为93.52%、90.75%和87.61%,P均<0.05。流式细胞术结果显示,PBS组和上述抗体处理组的细胞凋亡率分别为3.47%±0.36%、11.72%±2.03%、15.52%±3.05%和21.59%±4.49%,抗体处理组的细胞凋亡率均较PBS组高,P均<0.05。[结论]MMP14表达能显著促进TCa8113细胞的增殖和抗凋亡能力。MMP14单克隆抗体能显著抑制TCa8113细胞的增殖和抗凋亡能力。

[Objective]To explore the effect of MMP14 monoclonal antibody on the proliferation and apoptosis of oral squamous cell carcinoma cells.[Method]MMP14 knockdown(Kd-TCa8113)and overexpression(Kd-TCa8113)cell lines were constructed and compared with wild-type TCa8113(Wt-TCa8113)to investigate the effect of MMP14 on the proliferation and apoptosis of TCa8113 cells.Different concentrations of MMP14 monoclonal antibody and TCa8113 were co-cultured to explore the effect of MMP14 monoclonal antibody on the proliferation and apoptosis of TCa8113 cells.[Result]Taking Wt-TCa8113 as the control group,the proliferation rates of Kd-TCa8113 group and Kd-TCa8113 group were 74.55%and 114.79%respectively after 48 hours of culture,P<0.05.The results of flow cytometry showed that the apoptosis rates of Wt-TCa8113,Kd-TCa8113,and Oe-TCa8113 were 3.47%±0.36%,33.68%±7.33%,and 1.60%±0.22%after cultured for 48 hours,P<0.05.After treating TCa8113 cells with 50μg/mL,100μg/mL,and 200μg/mL antibodies for 24 hours,taking the PBS treatment group as the control,the cell proliferation rates were 93.52%,90.75%,and 87.61%,respectively,P<0.05.The results of flow cytometry showed that the apoptosis rates of the PBS group and the above antibody treatment groups were 3.47%±0.36%,11.72%±2.03%,15.52%±3.05%,and 21.59%±4.49%,respectively.Compared with the PBS group,P<0.05.[Conclusion]The expression of MMP14 can significantly promote the proliferation and anti-apoptotic ability of TCa8113 cells.MMP14 monoclonal antibody can significantly inhibit the proliferation and anti-apoptotic ability of TCa8113 cells.