KLK11对胃癌细胞增殖、迁移和侵袭的影响及预后分析

Effect of KLK11 on proliferation,migration,and invasion of gastric cancer cells and its prognosis

[目的]探索KLK11对胃癌细胞SGC7901的增殖、迁移、侵袭的影响,及KLK11与胃癌预后的关系。[方法]采用QRT-PCR检测24例胃癌组织和配对癌旁组织中KLK11 mRNA的表达。37℃、5%CO_(2)培养不同胃癌细胞株(GES-1、AGS、HGC-27、SCG7901),采用Lipofectamine2000转染试剂转染pcDNA3.1-KLK11质粒至不同胃癌细胞株,通过Western Blotting检测KLK11蛋白在不同胃癌细胞株中的不同表达水平,选择SGC7901细胞株继续后续实验。通过CCK-8实验和克隆形成实验检测KLK11过表达细胞株SGC7901的增殖情况,采用transwell实验检测过表达KLK11对SGC7901细胞迁移侵袭的影响。通过Kaplan-Meier生存曲线分析TCGA数据库中KLK11 mRNA表达水平与胃癌预后的关系。[结果]KLK11 mRNA在胃癌组织中表达水平明显低于癌旁组织组,差异有统计学意义(P<0.05)。过表达组KLK11表达高于空白组和对照组,差异有统计学意义(P<0.05)。过表达组增殖、迁移和侵袭能力低于空白组和对照组,差异有统计学意义(P<0.05)。TCGA数据显示,胃癌KLK11 mRNA高表达组较低表达组预后更差(P<0.05)。[结论]过表达KLK11抑制胃癌细胞的增殖、迁移和侵袭,胃癌低表达KLK11是胃癌患者预后不良的因素之一,说明KLK11可能是治疗胃癌的潜在靶点。

[Objective] To explore the biofunction of KLK11 in gastric cancer and the relationship with prognosis.[Method]QRT-PCRwas used to detect mRNA of KLK11 in gastric cancer tissues and the paired adjacent tissues.In 37℃,5%CO_(2) incubator,plasmid pcDNA3.1-KLK11 was transfected into different gastric cancer cell lines(GES-1,AGS,HGC-27,SCG7901) via Lipofectamine2000.And Western Blotting was used to detect levels of KLK11 in different gastric cancer cell lines.SCG7901 was selected to the following experiments.The proliferation of KLK11 overexpressing cell line SGC7901 was examined by CCK-8 assay and clone formation assay,and the effect of KLK11 overexpression on the migration and invasion of SGC7901 cells was examined by transwell assay.Kaplan-Meier survival curve was used to analyze the relationship between KLK11 mRNA expression and prognosis of gastric cancer in the TCGA database.[Result]The expression level of KLK11 mRNA in gastric cancer was significantly lower than that in adjacent tissues(P<0.05).The expression of KLK11 in overexpression group was significantly higher than that in blank group and control group(P<0.05).The ability of proliferation,migration and invasion in overexpression group was lower than that in blank group,and the difference was statistically significant(P<0.05).TCGA data showed that the prognosis of gastric cancer KLK11 mRNA high expression group was worse than that of low expression group(P<0.05).[Conclusion] Overexpression of KLK11 inhibits the proliferation,migration,and invasion of gastric cancer cells,and low expression of KLK11 is a key factor for poor prognosis in gastric cancer patients.It implicit that KLK11 may be a potential therapy target for gastric cancer.