新疆和静县黑头羊犬新孢子虫的检测及分子鉴定

Detection and Molecular Identification of Neos pora Caninum in Scapegoat in Hejing County

犬新孢子虫是一种细胞内原生动物寄生虫,可导致包括绵羊和山羊在内的多种哺乳动物流产和新生胎儿死亡。本研究为了预估巴音郭楞蒙古自治州和静县黑头羊中犬新孢子虫的流行情况,采集了8个乡镇的165份血样,运用聚合酶链式反应(PCR)扩增犬新孢子虫Nc5基因片段,并对部分阳性产物进行基因测序。结果显示,在165只黑头羊中有13只(7.9%)检测到犬新孢子虫阳性;年龄、流产史、饲养犬只与感染率有统计学差异(P<0.05);2岁以上的母羊感染率最高,有流产史的母羊,新孢子虫感染率较高。饲养场地周边犬的存在对感染率也有所影响,有饲养犬的羊群感染率11.4%,高于未饲养犬的羊群。将Nc5基因的部分序列与GenBank上传的基因序列进行比对,结果显示同源性为92%~100%。本研究是首次对和静县黑头羊犬新孢子虫进行系统进化分析,为犬新孢子虫病防控方案的制订提供参考,对减少畜牧业的经济损失具有实际意义。

Neos pora caninum is an intracellular protozoan parasite that can cause abortion and neonatal fetal death in a variety of mammals,including sheep and goats.The purpose of this study was to estimate the prevalence of neosporoasis in scapegoat in Hejing County,Bayingolin Mongolian Autonomous Prefecture.PCR was used to amplify the NC5 gene fragment of neosporoasis canine,and some positive products were sequenced.Results Showed that 13 of 165 scapegoat(7.9%)were positive for canine N.caninum.Age,abortion history,dog ownership and infection rate were significantly different(P<0.05).The infection rate of ewes over 2 years old was the highest.In addition,the infection rate of neosporoasis was higher in ewes with a history of abortion,and the presence of dogs around the breeding site also affected the infection rate,and the infection rate of sheep with dogs 11.4%,was higher than that of sheep without dogs.The partial sequence of NC5 gene was compared with that of NC5 gene stored in GenBank,and the homology of NC5 gene was 92%~100%.To our knowledge,this is the first phylogenetic analysis of N.caninum in scapegoat from Hejing county.This information is important for designing control schemes to reduce the economice losses of animal husbandry.