萱草花药材DNA条形码鉴别与HPLC指纹图谱研究

DNA Barcoding Identification and HPLC Fingerprint of Hemerocallis citrina

目的:利用DNA条形码技术对萱草花药材进行物种鉴定,建立其指纹图谱,为萱草花质量控制和鉴定提供依据。方法:提取14批样品的DNA进行序列分析和物种鉴定,并建立高效液相色谱法指纹图谱,采用Waters HSS T3型色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.2%乙酸为流动相,梯度洗脱,流速为1 mL·min^(–1),检测波长为360 nm,柱温为35℃。结果:经DNA条形码鉴定,14批萱草花基原均为黄花菜Hemerocallis citrina Baroni。结合指纹图谱共标定了14个共有峰。结论:DNA条形码和指纹图谱2种方法可对萱草花进行物种鉴定,并能全面反映萱草花药材中的成分信息,可用于萱草花药材的真伪鉴定和批间一致性评价。

Objective:To identify the species of Hemerocallis citrina by DNA barcoding technology and establish its fingerprint,providing a basis for quality control and identification of H.citrina.Methods:The DNA of 14 batches of samples was extracted for sequence analysis and species identification.The high performance liquid chromatography(HPLC)fingerprint was established.Waters HSST3 column(250 mm×4.6 mm,5μm)was used,with acetonitrile-0.2%acetic acid solution as mobile phase for gradient elution.The flow rate was 1 ml/min,the detection wavelength was 360 nm,and the column temperature was 35℃.Results:After DNA barcoding identification,all 14 batches of H.citrina flower were identified.On this basis,HPLC fingerprints of 14 batches of samples were established and 14 common peaks were calibrated.Conclusion:DNA barcoding and fingerprint can be used to identify H.citrina flower species and reflect the composition information of H.citrina,which can be used for identification of H.citrina and evaluation of consistency among batches.