Lipidomic investigation of the protective effects of Polygonum perfoliatum against chemical liver injury in mice

Objective:Recent investigations have demonstrated that Polygonum perfoliatum L.can protect against chemical liver injury,but the mechanism behind its efficacy is still unclear.Therefore,we studied the pharmacological mechanism at work in P.perfoliatum protection against chemical liver injury.Methods:To evaluate the activity of P.perfoliatum against chemical liver injury,levels of alanine transaminase,lactic dehydrogenase,aspartate transaminase,superoxide dismutase,glutathione peroxidase and malondialdehyde were measured,alongside histological assessments of the liver,heart and kidney tissue.A nontargeted lipidomics strategy based on ultra-performance liquid chromatography quadrupoleorbitrap high-resolution mass spectrometry method was used to obtain the lipid profiles of mice with chemical liver injury and following treatment with P.perfoliatum;these profiles were used to understand the possible mechanisms behind P.perfoliatum's protective activity.Results:Lipidomic studies indicated that P.perfoliatum protected against chemical liver injury,and the results were consistent between histological and physiological analyses.By comparing the profiles of liver lipids in model and control mice,we found that the levels of 89 lipids were significantly changed.In animals receiving P.perfoliatum treatment,the levels of 8 lipids were significantly improved,relative to the model animals.The results showed that P.perfoliatum extract could effectively reverse the chemical liver injury and significantly improve the abnormal liver lipid metabolism of mice with chemical liver injury,especially glycerophospholipid metabolism.Conclusion:Regulation of enzyme activity related to the glycerophospholipid metabolism pathway may be involved in the mechanism of P.perfoliatum's protection against liver injury.