摘要
目的:建立同时测定贞芪扶正颗粒中4种成分含量的高效液相色谱法。方法:采用HPLC法,色谱柱为Agilent Poroshell 120 EC-C 18(4.6mm×150 mm,4μm),流动相为乙腈-水,梯度洗脱,检测波长为216 nm,体积流量为1 mL·min^(-1),柱温为30℃,进样量为10μL。结果:红景天苷、酪醇、芦丁和特女贞苷质量浓度分别在0.524~52.4μg·L^(-1)、0.908~90.8μg·L^(-1)、1.656~165.6μg·L^(-1)和8.376~837.6μg·L^(-1)范围内与峰面积呈良好的线性关系,其相关系数分别为0.9997、0.9997、0.9996和0.9993,平均加样回收率分别为96.58%、101.21%、99.47%和102.53%;RSD分别为3.56%、1.29%、2.67%和3.68%。结论:该分析方法适用于贞芪扶正颗粒中特女贞苷、红景天苷、芦丁和酪醇的含量测定。
Objective To establish an HPLC method for simultaneous determination of four components in Zhen Qi Fu Zheng granules.Methods The chromatographic column was Agilent poroshell 120 ec-C 18(4.6 mm×150 mm,4μm),the mobile phase acetonitrile water,gradient elution;The detection wavelength is 216 nm;The volume flow rate is 1 mL·min^(-1),The column temperature is 30℃,and the injection volume is 10μL.Results The mass concentrations of Salidroside,Tyrosol,Rutin and Ligustrum lucidum glycoside showed a good linear relationship with the peak area in the range of 0.524-52.4μg·L^(-1),0.908-90.8μg·L^(-1),1.656^(-1)65.6μg·L^(-1) and 8.376-837.6μg·L^(-1),respectively.The correlation coefficients were 0.9997,0.9997,0.9996 and 0.9993,and the average recoveries were 96.58%,101.21%,99.47%and 102.53%respectively;RSD was 3.56%,1.29%,2.67%and 3.68%respectively.Conclusion The analytical method established in this experiment is applicable to the determination of Ligustrum lucidum glycoside,salidroside,rutin and tyrosol in Zhen Qi Fu Zheng granules.
作者
贾安
茹国华
黄小强
黄涛
王彩霞
张世宇
张廷茹
JIA An;RU Guohua;HUANG Xiaoqiang;HUANG Tao;WANG Caixia;ZHANG Shiyu;ZHANG Tingru(Medical School,Huanghe Science&Technology College,Zhengzhou 450063,China;College of Pharmacy,Henan Uinversity,Kaifeng 475004,China)
出处
《中国民族民间医药》
2023年第10期34-38,共5页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
郑州市抗肿瘤中药研究重点实验室项目资助
郑州市医药创新科技服务平台(YYCXPT2016)
2021年河南省级大学生创新创业训练计划项目(S202111834003)
黄河科技学院大学生创新创业训练计划(2021xscxcy006)。
关键词
贞芪扶正颗粒
HPLC
含量测定
Zhen Qi Fu Zheng Granules
HPLC
Content Determination