HPLC法测定贞芪扶正颗粒中4个成分的含量

Determination of Four Components in Zhen Qi Fu Zheng Granules by HPLC

目的:建立同时测定贞芪扶正颗粒中4种成分含量的高效液相色谱法。方法:采用HPLC法,色谱柱为Agilent Poroshell 120 EC-C 18(4.6mm×150 mm,4μm),流动相为乙腈-水,梯度洗脱,检测波长为216 nm,体积流量为1 mL·min^(-1),柱温为30℃,进样量为10μL。结果:红景天苷、酪醇、芦丁和特女贞苷质量浓度分别在0.524~52.4μg·L^(-1)、0.908~90.8μg·L^(-1)、1.656~165.6μg·L^(-1)和8.376~837.6μg·L^(-1)范围内与峰面积呈良好的线性关系,其相关系数分别为0.9997、0.9997、0.9996和0.9993,平均加样回收率分别为96.58%、101.21%、99.47%和102.53%;RSD分别为3.56%、1.29%、2.67%和3.68%。结论:该分析方法适用于贞芪扶正颗粒中特女贞苷、红景天苷、芦丁和酪醇的含量测定。

Objective To establish an HPLC method for simultaneous determination of four components in Zhen Qi Fu Zheng granules.Methods The chromatographic column was Agilent poroshell 120 ec-C 18(4.6 mm×150 mm,4μm),the mobile phase acetonitrile water,gradient elution;The detection wavelength is 216 nm;The volume flow rate is 1 mL·min^(-1),The column temperature is 30℃,and the injection volume is 10μL.Results The mass concentrations of Salidroside,Tyrosol,Rutin and Ligustrum lucidum glycoside showed a good linear relationship with the peak area in the range of 0.524-52.4μg·L^(-1),0.908-90.8μg·L^(-1),1.656^(-1)65.6μg·L^(-1) and 8.376-837.6μg·L^(-1),respectively.The correlation coefficients were 0.9997,0.9997,0.9996 and 0.9993,and the average recoveries were 96.58%,101.21%,99.47%and 102.53%respectively;RSD was 3.56%,1.29%,2.67%and 3.68%respectively.Conclusion The analytical method established in this experiment is applicable to the determination of Ligustrum lucidum glycoside,salidroside,rutin and tyrosol in Zhen Qi Fu Zheng granules.