环状RNA hsa_circ_0007813在乳腺癌组织的表达及其临床意义

Expression of circular RNA hsa_circ_0007813 in breast cancer tissues and its effect on prognosis of patients

目的探讨乳腺癌组织中环状RNA hsa_circ_0007813的表达水平及与患者临床病理特征及生存率间的关系。方法选取上海芯超生物科技有限公司的132例乳腺癌组织芯片作为研究对象。采用荧光原位杂交技术(FISH)检测环状RNA hsa_circ_0007813的表达,并分析hsa_circ_0007813的表达与患者临床病理特征及患者预后间的关系。采用χ^(2)检验分析hsa_circ_0007813与临床病理特征间的关系,利用Kaplan-Meier方法评估患者的总生存率,采用Cox回归模型进行多因素分析。结果FISH检测结果显示,乳腺癌组织中hsa_circ_0007813表达阳性率71.21%(94/132)明显高于癌旁组织28.79%(38/132)(χ^(2)=47.515,P<0.05),病理分期Ⅲ~Ⅳ期患者乳腺癌组织hsa_circ_0007813的表达阳性率69.88%(58/83)明显高于Ⅰ~Ⅱ期患者乳腺癌组织hsa_circ_0007813的表达阳性率51.02%(25/49)(χ^(2)=4.695,P<0.05),发生淋巴结转移的患者hsa_circ_0007813表达阳性率81.01%(64/79)明显高于未发生转移的患者56.60%(30/53)(χ^(2)=9.219,P<0.05)。Kaplan-Meier生存分析结果显示,hsa_circ_0007813高表达组患者生存率73.40%(69/94)明显低于hsa_circ_0007813低表达组患者生存率97.37%(37/38)(χ^(2)=8.930,P<0.05)。多因素回归分析结果显示临床分期[风险比(HR)=3.246,95%可信区间(CI):1.273~8.368,P<0.05]和hsa_circ_0007813表达水平(HR=3.540,95%CI:1.053~11.903,P<0.05)均为影响乳腺癌患者总生存率的独立危险因素。结论环状RNA hsa_circ_0007813在乳腺癌组织中呈高表达,与患者病理分期、淋巴结转移及患者生存率明显相关。

Objective To observe the expression level of circular RNA hsa_circ_0007813 in breast cancer tissues and its relationship with clinicopathologic features and survival rate of patients.Methods A total of 132 cases of breast cancer tissue microarray were collected in Shanghai Outdo Biotech as the research objects.Fluorescence in situ hybridization(FISH)was used to detect the expression of hsa_circ_0007813,and to analyze the relationship between the expression of hsa_circ_0007813 and the clinicopathological characteristics and prognosis of patients.Chi-square test was used to analyze the relationship between hsa_circ_0007813 and clinicopathological features.Kaplan-Meier method was used to evaluate the overall survival rate of patients.Cox regression model was used for multivariate analysis.Results FISH results showed that the positive rate of hsa_circ_0007813 expression in breast cancer tissues was 71.21%(94/132),which was significantly higher than that in adjacent tissues[28.79%(38/132),χ^(2)=47.515,P<0.05].The positive rate of hsa_circ_0007813 expression in breast cancer tissues of patients with pathological stagesⅢ-Ⅳwas 69.88%(58/83),which was significantly higher than 51.02%(25/49)in breast cancer tissues of patients with stageⅠ-Ⅱ(χ^(2)=4.695,P<0.05).The positive rate of hsa_circ_0007813 expression in patients with lymph node metastasis was 81.01%(64/79),which was significantly higher than 56.60%(30/53)in patients without lymph node metastasis(χ^(2)=9.219,P<0.05).Kaplan-Meier survival analysis showed that the survival rate of patients in high hsa_circ_0007813 expression group was 73.40%(69/94),which was significantly lower than that in low hsa_circ_0007813 expression group[97.37%(37/38),χ^(2)=8.930,P<0.05].Multivariate regression analysis showed clinical stage[hazard ratio(HR)=3.246,95%confidence interval(CI):1.273-8.368,P<0.05]and expression level of hsa_circ_0007813(HR=3.540,95%CI:1.053-11.903,P<0.05)were independent risk factors affecting the overall survival rate of breast cancer patients.Conclusion Circular RNA hsa_circ_0007813 was highly expressed in breast cancer tissue,which was significantly related to the pathological stage,lymph node metastasis and survival rate of patients.