嗜麦芽窄食单胞菌对肺腺癌A549细胞系转录组基因表达的影响

Effect of Stenotrophomonas maltophilia on transcriptome gene expression of lung adenocarcinoma A549 cell line

目的探讨嗜麦芽窄食单胞菌对肺腺癌细胞转录组基因表达的影响,分析可能的目标基因。方法实验分为对照组和嗜麦芽窄食单胞菌组,嗜麦芽窄食单胞菌作用于A549细胞系4 h后利用转录组学测序分析基因表达,筛选差异表达的基因,通过基因本体(GO)富集分析及京都基因与基因组百科全书KEGG富集分析,并且使用Broad Institute开发的基因集富集分析(GSEA)算法进行分析,帮助识别所选择的样本中表达量变化最明显的基因集中在哪些通路、生物学过程或功能组分等。结果经过限定条件的筛选,共有2285个基因差异表达,其中上调的基因有1669个,下调的基因有616个,其中HDAC5在S.maltophilia中显著上调。KEGG富集分析发现丝裂原活化蛋白激酶(MAPK)信号通路、p53信号通路等在S.maltophilia组与对照组之间差异有统计学意义,通过GSEA分析酪氨酸激酶(JAK)-信号转导与转录激活因子(STAT)信号通路、MAPK信号通路、Ras信号通路、磷脂酰肌醇3激酶(PI3K)-蛋白激酶B(Akt)信号通路等在S.maltophilia组中显著上调通过KDA分析,CXC型趋化因子配体8(CXCL8)在S.maltophilia组中显著上调通过。结论CXCL8可能通过上调PI3K-Akt信号通路发挥S.maltophilia促进肺腺癌A549细胞增殖的作用。

Objective The effect of stenotrophomonas maltophilia on the gene expression of lung adenocarcinoma cells was explored through the differential expression of the gene of lung cancer A549 cells treated by Stenotrophomonas maltophilia and functional analysis.Methods The experiment was divided into control group and stenotrophomonas maltophilia group.At 4 h after the treatment of stenotrophomonas maltophilia on A549 cell line,transcriptomic sequencing was used to analyze the changes in gene expression,and the differentially expressed genes were screened by gene ontology(GO)enrichment analysis and KEGG enrichment analysis of Kyoto Encyclopedia of Genes and Genomes.In addition,the gene set enrichment analysis(GSEA)algorithm developed by Broad Institute was used for analysis to help identify which pathways,biological processes or functional components of genes with the most obvious expression changes in the selected samples were concentrated.Results After screening with limited conditions,we found that a total of 2285 genes were differentially expressed,among which there were 1669 up-regulated genes and 616 down-regulated genes.HDAC5 was significantly upregulated in S.maltophilia.KEGG enrichment analysis showed that mitogen-activated protein kinase(MAPK)signaling pathway and p53 signaling pathway were different between S.maltophilia group and control group.The GSEA analysis revealed that janus kinase(JAK)-signal transducers and activators of transcription(STAT)signaling pathway,MAPK signaling pathway,Ras signaling pathway and phosphatidylinositol 3 kinase(PI3K)-protein kinase B(Akt)signaling pathway were significantly up-regulated in S.maltophilia group.The KDA analysis indicated that CXC tchemokine ligand 8(CXCL8)was significantly up-regulated in the S.maltophilia group.Conclusion CXCL8 may play the role of S.maltophilia in promoting the proliferation of lung adenocarcinoma A549 cells by up-regulating the PI3K-Akt signal pathway.