云南美登木愈伤组织化学成分的研究

Chemical constituents from the calli of Maytenus hookeri

采用RP-18中压柱、硅胶柱和Sephadex LH-20柱等色谱技术对云南美登木愈伤组织的干燥细胞和新鲜细胞提取物的乙酸乙酯部分进行分离纯化得到16个化合物。通过核磁共振和质谱等波谱技术鉴定化学结构为雷公藤内酯甲(1),雷公藤内酯乙(2),3β,22α-二羟基齐墩果-12-烯-29-酸(3),22α-羟基-3-氧-齐墩果-12-烯-29-酸(4),3-表卡通酸(5),齐墩果-9(11),12-二烯-3-β-醇(6),22α-羟基-3-氧-乌苏-12-烯-30-酸(7),雷公藤三萜酸C(8),2α,3β-二羟基-木栓烷-29-酸(9),美登酮酸(10),3β-羟基豆甾-5-烯-7-酮(11),7β-羟基谷甾醇(12),豆甾-4-烯-3-酮(13),β-谷甾醇(14),1-β-D-glucpotyransyl-2,6-dimetoxi-4-propenyl-benzen(15)和腺苷(16)。除化合物10和14外,其余化合物均为首次从云南美登木植物细胞及其植株中分离得到。

In order to study the chemical compounds of the calli induced from Maytenus hookeri,the dried and fresh cells of the calli were extracted by heat refluxing and cold soaking method,respectively.The chemical constituents were purified through various column chromatography including silica gel,RP-18 and Sephadex LH-20 from the ethyl acetate extract.According to the NMR and MS analysis,sixteen compounds were identified as wilforlide A(1),wilforlide B(2),3β,22α-dihydroxyolean12-en-29-oic acid(3),22α-hydroxy-3-oxoolean-12-en-29-oic acid(4),3-epikatonic acid(5),oleana-9(11),12-dien-3-β-ol(6),22α-hydroxy-3-oxo-12-ursen-30-oic acid(7),triptotriterpenic acid C(8),2α,3β-dihydroxy-29-friedelanoic acid(9),maytenonic acid(10),3β-hydroxystigmast-5-en-7-one(11),7β-hydroxysitosterol(12),stigmast-4-ene-3-one(13),β-sitosterol(14),1-β-Dglucpotyransyl-2,6-dimetoxi-4-propenyl-benzene(15)and adenosine(16).All compounds were reported in the calli of M.hookeri and the plant for the first time except 10 and 14.