黄芩苷对猪源肠外致病性大肠杆菌的体内保护作用

Protective effect of baicalin on porcine extra-intestinal pathogenic Escherichia coli in vivo

为探究黄芩苷对猪源肠外致病性大肠杆菌(Extraintestinal pathogenic Escherichia coli,ExPEC)感染小鼠后的保护作用,采用小鼠存活率试验和组织载菌量试验评估黄芩苷对小鼠的保护作用,比较感染组和黄芩苷治疗组小鼠临床表现、存活率、组织载菌量及病理变化。结果表明,猪源肠外致病性大肠杆菌感染后小鼠状态萎靡不振、食欲下降,黄芩苷治疗组小鼠状态较感染组状态好;黄芩苷治疗组小鼠存活率相对于感染组有一定提升;黄芩苷治疗组小鼠心脏、肝脏、脾脏和肾脏中组织载菌量及病理变化相对于感染组均极显著降低(P<0.01)。

To explore the protective effect of baicalin on mice infected with porcine extraintestinal pathogenic Escherichia coli,the survival rate assay and colonization assay of mice were selected to evaluate the protective effect of baicalin.The clinical feature,survival rate,tissue bacterial load and pathological changes of mice in the infected group and baicalin treatment group were compared.The results showed that the mental state of mice treated by baicalin was better than that of the mice infected by PCN033.The survival rate of the baicalin treatment group was improved compared with that of the infection group.Bacterial counts in heart,liver,spleen and kidney of mice treated with baicalin were significantly reduced compared with those of mice infected with PCN033(P<0.01).