基于转录组挖掘不同碳源条件下解淀粉芽孢杆菌TF28脂肽合成相关基因

Identification of genes related to lipopeptide synthesis in mining lipopeptide synthesis related genes of Bacillus amyloliquefaciens TF28 under different carbon sources based on transcriptome

以解淀粉芽孢杆菌(Bacillus amyloliquefaciens)TF28为供试菌株,设置葡萄糖组(对照)、果糖组、木糖组3个实验组,通过转录组测序鉴定差异基因,分别对差异基因进行功能分析,挖掘脂肽合成调控基因。果糖组共鉴定到差异基因688个,上调基因522个,下调基因166个;木糖组共鉴定到差异基因855个,上调基因691个,下调基因164个。不同碳源改变了解淀粉芽孢杆菌TF28脂肽合成群体感应系统、双组分系统全局调控因子的表达水平,并影响脂肽必需氨基酸及脂肪酸的合成代谢,为进一步研究脂肽合成的生物学调控机制提供参考。

With Bacillus amyloliquefaciens TF28 as the test strain,three experimental groups were set up:glucose group(control),fructose group and xylose group.Differential genes were identified by transcriptome sequencing,and functional analysis was carried out on the differential genes to explore the regulatory genes for lipopeptide synthesis.A total of 688 differentially expressed genes,522 upregulated genes,and 166 downregulated genes were identified in the fructose group;a total of 855 differential genes,691 upregulated genes,and 164 downregulated genes were identified in the xylose group.Different carbon sources changed the expression levels of global regulatory factors in the quorum sensing system and dual component system of lipopeptide synthesis in starch Bacillus subtilis TF8,and influenced the synthesis and metabolism of essential amino acids and fatty acids in lipopeptide synthesis,which provided reference for further research on the biological regulatory mechanisms of lipopeptide synthesis.